Homocysteine Assay Kit (Colorimetric) Summary
Description |
Detection instrument: Microplate reader (330 - 350 nm, optimum wavelength: 340 nm) Assay Time: 25 min |
Standard Curve Range |
1.95 - 100.0 umol/L |
Sensitivity |
1.95 umol/L |
Inter-Assay |
%CV < 9 |
Intra-Assay |
%CV < 1.7 |
Spike Recovery |
Average recovery rate: 104% |
Kit Type |
Assay Kit (Colorimetric) |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- 28 umol/L Standard
- Buffer Solution A
- Buffer Solution B
- Enzyme Reagent
- Substrate
|
Alternate Names for Homocysteine Assay Kit (Colorimetric)
Background
Homocysteine is a chemical compound with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the naturally-occurring amino acid cysteine, differing in that its side-chain contains an additional methylene (-CH2-) group before the thiol (-SH) group. Alternatively, Homocysteine can be derived from methionine by removing the latter's terminal C methyl group. Elevations of Homocysteine also occur in the rare hereditary disease homocystinuria and in methylene-tetrahydrofolate-reductase deficiency. The latter is quite common and usually goes unnoticed, although there are reports that thrombosis and cardiovascular disease occurs more often in people with elevated Homocysteine.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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