Homocysteine Assay Kit (Colorimetric)

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Homocysteine Assay Kit (Colorimetric) [NBP3-25825] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum (plasma) and urine
Standard Curve Range
1.95 - 100.0 umol/L
Sensitivity
1.95 umol/L

Order Details

Homocysteine Assay Kit (Colorimetric) Summary

Description
Detection instrument: Microplate reader (330 - 350 nm, optimum wavelength: 340 nm)
Assay Time: 25 min
Standard Curve Range
1.95 - 100.0 umol/L
Sensitivity
1.95 umol/L
Inter-Assay
%CV < 9
Intra-Assay
%CV < 1.7
Spike Recovery
Average recovery rate: 104%
Kit Type
Assay Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 28 umol/L Standard
  2. Buffer Solution A
  3. Buffer Solution B
  4. Enzyme Reagent
  5. Substrate

Alternate Names for Homocysteine Assay Kit (Colorimetric)

  • (2S) 2 amino 4 sulfanyl butanoic acid

Background

Homocysteine is a chemical compound with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the naturally-occurring amino acid cysteine, differing in that its side-chain contains an additional methylene (-CH2-) group before the thiol (-SH) group. Alternatively, Homocysteine can be derived from methionine by removing the latter's terminal C methyl group. Elevations of Homocysteine also occur in the rare hereditary disease homocystinuria and in methylene-tetrahydrofolate-reductase deficiency. The latter is quite common and usually goes unnoticed, although there are reports that thrombosis and cardiovascular disease occurs more often in people with elevated Homocysteine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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