Recombinant Human HP1 alpha His Protein

SDS-Page: Recombinant Human HP1 alpha Protein [NBP1-98863] - 15% SDS-PAGE (3 ug).

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human HP1 alpha His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-191 of Human HP1 alpha
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGKKTK RTADSSSSED EEEYVVEKVL DRRVVKGQVE YLLKWKGFSE EHNTWEPEKN LDCPELISEF MKKYKKMKEG ENNKPREKSE SNKRKSNFSN SADDIKSKKK REQSNDIARG FERGLEPEKI IGATDSCGDL MFLMKWKDTD EADLVLAKEA NVKCPQIVIA FYEERLTWHA YPEDAENKEK ETAKS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CBX5
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 24.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 30% glycerol, 0.1 M NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HP1 alpha His Protein

• Antigen p25
• chromobox homolog 5 (Drosophila HP1 alpha)
• chromobox homolog 5 (HP1 alpha homolog, Drosophila)
• chromobox homolog 5
• chromobox protein homolog 5
• Heterochromatin protein 1 homolog alpha
• heterochromatin protein 1-alpha
• HP1 alpha homolog
• HP1 alpha
• HP1
• HP1A
• HP1-ALPHA
• HP1Hs alpha
• HP1Hs-alpha

Background

Chromobox homolog 5, also known as CBX5, is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. CBX5 is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. It has a pseudogene located on chromosome 3. Also, The CBX5 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated. Recombinant human CBX5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CBX5