HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) [Biotin]

• Reactivity: V-Vi
• Applications: ICC/IF, IHC
• Clone: HPV16/1295 + HPV18/1297
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin

HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) [Biotin] Summary

• Immunogen: HPV18 E6-beta-galactosidase fusion protein
• Localization: Nuclear
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Type 16 of Human Papilloma Virus (HPV-16)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for HPV16/HPV18 E6 Antibody (HPV16/1295 + HPV18/1297) [Biotin]

• E6
• Human Papilloma Virus
• Human papillomavirus type 16 E6
• Human papillomavirus type 18 E6
• Protein E6

Background

Human Papilloma virus (HPV) is responsible for the development of type and tissue specific papilloma in the oral cavity and in the larynx. In the skin papilloma virus can cause different types of warts and in the ano-genital region it is associated with condyloma or carcinoma. HPV types 1, 6 and 11 usually are associated with benign transformations of the tissues, while e.g. HPV types 16, 18 and 31 seem to be responsible for the development of some carcinoma like cervical carcinoma. The role of HPV in non-melanoic tumours of the skin is under discussion. Protein E6 is a Transcriptional transactivator, binding double stranded DNA. It has transforming activity inactivating, with E6-AP ubiquitin-protein ligase, the human TP53/p53 tumor suppressor protein by targeting it for degradation. Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.