HPV16 L1 Antibody (CamVir 1) - BSA Free

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF, IHC, IP, KD
• Clone: CamVir 1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HPV16 L1 Antibody (CamVir 1) - BSA Free Summary

• Immunogen: A beta galactosidase-L1 fusion protein purified by PAGE.
• Epitope: C terminus of HPV16 L1
• Localization: Virion. Host nucleus.
• Specificity: Cross reacts with HPV37. Reacts very strongly with biopsy specimins containing HPV16 or HPV33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV6 or HPV11.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature (PMID 24231739)
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated reported in scientific literature (PMID 32210347)
  • Western Blot 1:100-1:2000
• Application Notes: This HPV16 L1 (CamVir 1) antibody antibody reacts with a 56 kDa protein in cells infected with L1-vaccinia virus, the protein being present in a predominantly nuclear location.
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human Papillomavirus (HPV)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for HPV16 L1 Antibody (CamVir 1) - BSA Free

• HPV16 capsid protein
• HPV-16 capsid
• HPV16 L1
• HPV16 major capsid protein L1
• HPV-16
• Human papillomavirus type 16 L1
• Human papillomavirus type 16 major capsid protein L1
• Major capsid protein L1
• Major capsid protein

Background

HPV16 L1, also known as HPV-16, Major capsid protein L1, and Major Capsid Protein is a papillomavirus. Papillomaviruses are small nonenveloped tumorigenic DNA viruses that infect the basal cells of the epithelium. Papillomavirions contain two proteins, L1 and L2, which encapsulate a closed, circular, double-stranded DNA. The major capsid protein L1 specifically binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. Thus, the location of HPV16 L1 is largely nuclear. Furthermore, stable interactions have been observed between L1 and the minor capsid protein L2, which is necessary for viral genome encapsulation. Virtually all cervical cancers are caused by human papilloma infections, with types 16 and 18 being responsible for about 70 percent of all cases. Currently, B-cell epitopes from type 16-L1 derived peptides are being investigated in order to gain a better understanding of immune responses to this virus (PMID: 22979950).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPV16 L1 Antibody (NB100-2732)(17)

Publications using NB100-2732

• Pushparajah D Design of a DNA-Encoded Human Papilloma Virus-Like Particle Displaying a Vascular Endothelial Growth Factor Antagonistic Peptide for Characterization in Mammalian Cells Thesis 2022-01-01 (WB, Virus)
• Finke J, Mikulicic S, Loster AL et al. Anatomy of a viral entry platform differentially functionalized by integrins alpha 3 and alpha 6 Sci Rep 2020-03-24 [PMID: 32210347] (WB, KD, Human)
• Palaniyandi M, Kato T, Park EY. Expression of human papillomavirus 6b L1 protein in silkworm larvae and enhanced green fluorescent protein displaying on its virus-like particles. SpringerPlus. 2012-01-01 [PMID: 23961359] (WB, Virus)
• Hirano T. Alcohol Consumption and Oxidative DNA Damage International Journal of Environmental Research and Public Health 2011-07-01 [PMID: 21845164]
• Orozco J, Carter JJ, Koutsky LA, Galloway DA. Humoral immune response recognizes a complex set of epitopes on human papillomavirus type 6 l1 capsomers J Virol. 2005-08-01 [PMID: 16014913] (WB)
• Carter JJ, Wipf GC, Benki SF et al. Identification of a human papillomavirus type 16-specific epitope on the C-terminal arm of the major capsid protein L1. J Virol. 2003-11-01 [PMID: 14557648] (WB, ELISA)
• Benyacoub J, Hopkins S, Potts A et al. The nature of the attenuation of Salmonella typhimurium strains expressing human papillomavirus type 16 virus-like particles determines the systemic and mucosal antibody responses in nasally immunized mice. Infect Immun. 1999-07-01 [PMID: 10377159] (WB)
• Caparros-Wanderley W, Savage N, Hill-Perkins M et al. Intratype sequence variation among clinical isolates of the human papillomavirus type 6 L1 ORF: clustering of mutations and identification of a frequent amino acid sequence variant. J Gen Virol. 1999-04-01 [PMID: 10211973] (WB)
• Nardelli-Haefliger D, Roden RB, Benyacoub J et al. Human papillomavirus type 16 virus-like particles expressed in attenuated Salmonella typhimurium elicit mucosal and systemic neutralizing antibodies in mice. Infect Immun. 1997-08-01 [PMID: 9234794] (WB)
• Sasaqawa T, Inoue M, Lehtinen M et al. Serological responses to human papillomavirus type 6 and 16 virus-like particles in patients with cervical neoplastic lesions. Clin Diagn Lab Immunol. 1996-07-01 [PMID: 8807204] (ELISA)
• Park DS, Selvey LA, Kelsall SR et al. Human papillomavirus type 16 E6, E7 and L1 and type 18 E7 proteins produced by recombinant baculoviruses. J Virol Methods. 1993-12-31 [PMID: 8106603]
• Stering J, Stanley M, Gatward G et al. Production of human papillomavirus type 16 virions in a keratinocyte cell line. J Virol. 1990-12-01 [PMID: 2173795] (IHC-P)
• Zhou J, Crawford L, McLean L et al. Increased antibody responses to human papillomavirus type 16 L1 protein expressed by recombinant vaccinia virus lacking serine protease inhibitor genes. J Gen Virol. 1990-09-01 [PMID: 2170578] (WB)
• McLean CS, Churcher MJ, Meinke J et al. Production and characterisation of a monoclonal antibody to human papillomavirus type 16 using recombinant vaccinia virus. J Clin Pathol. 1990-06-01 [PMID: 2166093] (WB, IHC-P)
• Davies DH, Hill CM, Rothbard JB et al. Definition of murine T helper cell determinants in the major capsid protein of human papillomavirus type 16. J Glen Virol. 1990-11-01 [PMID: 1701481] (WB)
• Kirnbauer R, Booy F, Cheng N et al. Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic. Proc Natl Acad Sci USA. 1992-12-15 [PMID: 1334560] (WB)
• Finke J Structural and functional characterization of the HPV16 entry platform on the cell surface Thesis 2020-01-01 (WB)

Bioinformatics

• Entrez:
  • 1489082(Human)
• Uniprot:
  • P03101(Human)