HRP Conjugate Stabilizer/Diluent

ELISA: HRP Conjugate Stabilizer/Diluent [901-0005]

Product Discontinued

HRP Conjugate Stabilizer/Diluent Summary

• Description: The performance of HRP conjugates diminishes over time. There are a number of factors that contribute to loss of performance including:
  • Inactivation of HRP.
  • Instability of the bond which links HRP to the antibody.
  • Microbial attack.
  • Denaturation of the antibody.
  • Loss of performance accelerates with increasing temperature and with increasing dilution of the conjugates.
  For these reasons HRP conjugates are often kept in a concentrated form at 4^oC.
  
  The conjugate stabilizer/diluent is a proprietary multi-component reagent system that protects antibody-HRP conjugates from all of the negative factors noted above, thus ensuring the best possible performance in experiments performed at room temperature. Moreover, the ability to store HRP conjugates at working dilutions eliminates waste and improves consistency from experiment to experiment.
  
  In addition to its proprietary enzyme stabilizers, it contains antimicrobial agents as well as blockers to enhance signal to background in immunoassay applications.
• Specificity: Even some of the best known stabilizers perform either poorly or only modestly in standard accelerated stability trials at 37C. LifeXtend HRP stabilizer/diluent affords the greatest protection and can substantially extend the shelf life of HRP conjugates stored at 4C or at room temperature.

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Background

The performance of HRP conjugates diminishes over time. There are a number of factors that contribute to loss of performance including (i) inactivation of HRP, (ii) instability of the bond which links HRP to the antibody, (iii) microbial attack and (iv) denaturation of the antibody. Loss of performance accelerates with increasing temperature and with increasing dilution of conjugate. For these reasons HRP conjugates are often kept in a concentrated form at 4C. LifeXtend conjugate stabilizer/diluent is a proprietary multi-component reagent system that protects antibody-HRP conjugates from all of the negative factors noted above, thus ensuring the best possible performance in experiments performed at room temperature. Moreover, the ability to store HRP conjugates at working concentration eliminates waste and improves consistency from experiment to experiment. In addition to its proprietary enzyme stabilizers, LifeXtend contains antimicrobial agents as well as blockers to enhance signal to background in immunoassay applications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Publications for HRP Conjugate Stabilizer/Diluent (901-0005)(1)

Publication using 901-0005

• Torrente MP, Castellano LM, Shorter J Suramin Inhibits Hsp104 ATPase and Disaggregase Activity. PLoS One. 2014-10-09 [PMID: 25299406]

Review for HRP Conjugate Stabilizer/Diluent (901-0005) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer ELISA Other 07/23/2009

Summary

• Application: ELISA
• Sample Tested: Fecal hormone extract
• Species: Other
• Lot: 707