HSP10/EPF Antibody (M1.4)

Western Blot: Hsp10 Antibody (M1.4) [NB120-13526] - Western blot analysis of chlamydia-infected human HeLa cell lysate probed with SPA-781

Product Discontinued

HSP10/EPF Antibody (M1.4) Summary

• Immunogen: A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on C. trachomatis cpn10 (residues 54-69).
• Localization: Mitochondrial
• Specificity: This affinity purified detects a 15 kDa protein, corresponding to the apparent molecular mass of cpn10 on SDSPAGE immunoblots, in C. trachomatis infected cells. This will not react with the human or mouse cpn10 homolog nor with E. coli GroES.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPE1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.25 mg/ml
• Application Notes: EIA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 0.25 ug/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 9.9 kDa). Not tested in other applications.Optimal dilutions/con

Reactivity Notes

Cross-reacts with C. trachomatis.Does not cross-react with Human, Mouse or E. coli.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: phosphate buffered saline (PBS), 0.1mM PMSF
• Preservative: No Preservative
• Concentration: 0.9 mg/ml
• Purity: Protein G purified

Alternate Names for HSP10/EPF Antibody (M1.4)

• 10 kDa chaperonin
• 10 kDa heat shock protein, mitochondrial
• Chaperonin 10 Homolog
• Chaperonin 10
• CPN10HSP10
• Early-pregnancy factor
• EPF
• GroES
• heat shock 10kD protein 1 (chaperonin 10)
• heat shock 10kDa protein 1 (chaperonin 10)
• HSP10
• HSPE1

Background

Homologs of E. coli Cpn10 make up a family of small heat shock proteins with an approximate molecular mass of 10 kDa (Hsp10s). Cpn10 acts as a co-chaperone and interacts with members of the Hsp60 family (GroEL in E. coli) to promote proper folding of polypeptides. In chlamydia, Cpn10 is thought to interact with cHsp60, a reported immunopathological antigen, identifying Cpn10 as a potential contributor to an immunological response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPE1