HspA1B Antibody (3B7) - Azide and BSA Free

Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in NIH/3T3.

Immunocytochemistry/Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02] - Validation of the LCM/MS data from cellular protrusions using microscopy. Immunofluorescence (IF) of proteins identified by LCM/MS not expected to be within protrusions based on their known functions/localizations. HspA1B was observed in dCAD protrusions and TNTs. White arrows/arrowheads show punctates within dCAD/hCAD protrusions and GCs or within TNTs, respectively. Scale bars = 10 um. CAD = Cath.A differentiated neuronal cell line; dCAD = serum-strarved differentiated CAD; TNT = Tunneling nanotube.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866487/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. Antibody concentration 6 ug/ml.

Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in PC-12.

Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7 Analysis of HSPA1B expression in IMR-32.

Western Blot: HspA1B Antibody (3B7) [H00003304-M02] - HSPA1B monoclonal antibody (M02), clone 3B7. Analysis of HSPA1B expression in Raw 264.7.

Immunocytochemistry/Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of monoclonal antibody to HSPA1B on HeLa cell. Antibody concentration 10 ug/ml.

Proximity Ligation Assay: HspA1B Antibody (3B7) [H00003304-M02] - Analysis of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-HSPA1B mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

ELISA: HspA1B Antibody (3B7) [H00003304-M02] - Detection limit for recombinant GST tagged HSPA1B is approximately 0.03ng/ml as a capture antibody.

Immunocytochemistry/ Immunofluorescence: HspA1B Antibody (3B7) [H00003304-M02] - Validation of the LCM/MS data from cellular protrusions using microscopy. Immunofluorescence (IF) of proteins identified by LCM/MS (A–D) “expected” or (E–H) “not expected” to be within protrusions based on their known functions/localizations. (A) Cd47 & (B) Anxa2 are found in both hCAD/dCAD protrusions. (C) Tenm2 is found within hCAD protrusions & (D) Cobl in GCs. Interestingly, (E) Grk5 was observed in hCAD protrusions; (F) Hist1h3b protein in hCAD protrusions & GCs (G) Hspa1b in dCAD protrusions & TNTs & (H) Arg1 in dCAD protrusions, GCs, & TNTs. White arrows/arrowheads show punctates within dCAD/hCAD protrusions & GCs or within TNTs, respectively. Scale bars = 10 μm. IF of all 8 proteins corroborate the LCM/MS protein identification for each subtypes of protrusions. (I) 1198 unique proteins were identified by LCM/MS to be in protrusions & 169 were found to be exclusive to protrusions. 904 of the 1198 proteins found in protrusions & 87 out of 169 exclusive proteins had images in the HPA Subcell database. IF images for the proteins identified by LCM/MS in protrusions, present in the HPA Subcell database, were observed & catalogued as being observed or not in protrusions (Figure S2). On average 4.7% of proteins are found in protrusions in the HPA database, 13.6% are found in our LCM isolated protrusions & 23% were found in the “exclusive” LCM protrusions. The fold enrichment increases by 2.9% or 4.8% when we looked at proteins identified by LCM/MS in protrusions or exclusive to protrusions, respectively. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30866487), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, DB, ELISA, Func, ICC/IF, IHC
• Clone: 3B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

HspA1B Antibody (3B7) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: HSPA1B (NP_005336, 531 a.a. ~ 611 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLY
• Specificity: HSPA1B (3B7)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPA1B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. Use in Dot blot reported in scientific literature (PMID: 26344128).

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 26344128). Porcine reactivity reported in scientific literature (PMID: 24862986). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for HspA1B Antibody (3B7) - Azide and BSA Free

• FLJ54328
• Heat shock 70 kDa protein 1/2
• heat shock 70 kDa protein 1A/1B
• heat shock 70kD protein 1B
• heat shock 70kDa protein 1B
• HSP70.1/HSP70.2
• HSP70-1/HSP70-2
• HSP70-1B
• HSP70-2
• HSPA1
• HSPA1A

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HspA1B Antibody (H00003304-M02)(6)

Publications using H00003304-M02

• Gousset K, Gordon A, Kumar Kannan S, Tovar J. A novel Microproteomic Approach Using Laser Capture Microdissection to Study Cellular Protrusions Int J Mol Sci 2019-03-07 [PMID: 30866487] (ICC/IF, Mouse)
• Gagaoua M, Terlouw EMC, Picard B. et al. The study of protein biomarkers to understand the biochemical processes underlying beef color development in young bulls. Meat Sci 2017-07-18 [PMID: 28750331]
• Gagaoua M, Terlouw EMC, Boudjellal A, Picard B. Coherent correlation networks among protein biomarkers of beef tenderness: What they reveal. Journal of proteomics 2015-10-14 [PMID: 26344128] (WB, Cytometric Bead Assay Standard, Bovine)
• Picard B, Gagaoua M, Micol D et al. Inverse relationships between biomarkers and beef tenderness according to contractile and metabolic properties of the muscle. J Agric Food Chem 2014-08-24 [PMID: 25175407]
• Hossain MA, De Souza AI, Bagul A et al. HSP70, Peroxiredoxin-3 and -6 are upregulated during renal warm ischaemia in a donation after circulatory death model. J Proteomics. 2014-05-24 [PMID: 24862986] (WB, Porcine)
• Guillemin N, Bonnet M, Jurie C et al. Functional analysis of beef tenderness. J Proteomics 2011-08-09 [PMID: 21855665]

Bioinformatics

• Gene Symbol: HSPA1B
• Entrez:
  • 3304(Human)
• OMIM:
  • 603012(Human)
• Uniprot:
  • NP_005336()