HSPA8/HSC71/Hsc70 Antibody (1B5) - BSA Free

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Gp, Ha, Pm, Rb, Sh
• Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IP, MS
• Clone: 1B5
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

HSPA8/HSC71/Hsc70 Antibody (1B5) - BSA Free Summary

• Immunogen: Native hamster Hsc70 (Hsp73).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: HSPA8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Electron Microscopy 1:10-1:500
  • ELISA
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Simple Western 1:25
  • Western Blot 1:1000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Rat B103 cells as sample; separated by size; antibody dilution of 1:25; matrix was 12-230 kDa; detected by Chemiluminescence.
• Theoretical MW: 73 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Turtle (100%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for HSPA8/HSC71/Hsc70 Antibody (1B5) - BSA Free

• Heat shock 70 kDa protein 8
• heat shock 70kD protein 8
• heat shock 70kDa protein 8
• heat shock cognate 71 kDa protein
• heat shock cognate protein 54
• heat shock cognate protein, 71-kDa
• HSC54
• HSC70
• HSC70heat shock 70kd protein 10
• HSC71
• HSP71
• HSP73
• HSP73constitutive heat shock protein 70
• HSPA10
• HSPA8
• LAP1
• lipopolysaccharide-associated protein 1
• LPS-associated protein 1
• MGC131511
• MGC29929
• NIP71
• N-myristoyltransferase inhibitor protein 71

Background

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSPA8/HSC71/Hsc70 Antibody (NBP1-97868)(11)

Publications using NBP1-97868

• Shao L, Ning K, Wang J et al. The large nonstructural protein (NS1) of the human bocavirus 1 (HBoV1) directly interacts with Ku70, which plays an important role in virus replication in human airway epithelia Journal of virology 2021-12-08 [PMID: 34878919] (Human)
• Li PP, Itoh N, Watanabe M et al. Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens. J Virol. 2009-01-01 [PMID: 18945763]
• Lu HA, Sun TX, Matsuzaki T et al. Heat shock protein 70 interacts with aquaporin-2 and regulates its trafficking. J Biol Chem. 2007-09-01 [PMID: 17636261]
• Chen S, Brown IR. Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases. Cell Stress Chaperones. 2007-01-01 [PMID: 17441507]
• Tetzlaff J, Tanzer L, Jones KJ et al. Exogenous androgen treatment delays the stress response following hamster facial nerve injury. J Neuroendocrinol. 2007-05-01 [PMID: 17425613]
• Meinander A, Soderstrom TS, Kaunisto A et al. Fever-like hyperthermia controls T Lymphocyte persistence by inducing degradation of cellular FLIPshort. J Immunol. 2007-03-01 [PMID: 17339495]
• Suttitanamongkol S, Polanowska-Grabowska R, Gear AR et al. Heat-shock protein 90 complexes in resting and thrombin-activated platelets. Biochem Biophys Res Commun. 2002-09-01 [PMID: 12220519]
• Yamano T, Murata S, Shimbara N et al. Two distinct pathways mediated by PA28 and hsp90 in major histocompatibility complex class I antigen processing. J Exp Med. 2002-07-01 [PMID: 12119343]
• Bailey CK, Andriola IF, Kampinga HH et al. Molecular chaperones enhance the degradation of expanded polyglutamine repeat androgen receptor in a cellular model of spinal and bulbar muscular atrophy. Hum Mol Genet. 2002-03-01 [PMID: 11875046]
• Brighty DW, Jassal SR. The synthetic peptide P-197 inhibits human T-cell leukemia virus type 1 envelope-mediated syncytium formation by a mechanism that is independent of Hsc70. J Virol. 2001-11-01 [PMID: 11581416]
• Sainis L, Angelidis C, Pagoulatos GN et al. HSC70 interactions with SV40 viral proteins differ between permissive and nonpermissive mammalian cells. Cell Stress Chaperones. 2000-04-01 [PMID: 11147964]

Review for HSPA8/HSC71/Hsc70 Antibody (NBP1-97868) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer Simple Western Rat 01/05/2017

Summary

• Application: Simple Western
• Sample Tested: B103 Cells
• Species: Rat

Comments

• Comments: NBP1-97868, on rat B103 cells (~125, 250, 750, and 1500 cell equivalents per lane [left to right]) using 1:25 dilution of antibody and Jackson Immuno HRP-conjugated anti-rabbit (1:100 dil). The signal is clean at ~70 kDa, although there is cross reactivity with the 230-kDa fluorescent migration control.

Bioinformatics

• Gene Symbol: HSPA8