HSPA8/HSC71/Hsc70 Antibody (SR39-04)

Western Blot: HSPA8/HSC71/Hsc70 Antibody (SR39-04) [NBP2-67335] - Analysis of Hsc70 on different lysates using anti-Hsc70 antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: NIH/3T3 Lane 4: PC-12

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (SR39-04) [NBP2-67335] - Staining Hsc70 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (SR39-04) [NBP2-67335] - Analysis of paraffin-embedded human tonsil tissue using anti-Hsc70 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody (SR39-04) [NBP2-67335] - Staining Hsc70 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (SR39-04) [NBP2-67335] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Hsc70 antibody. Counter stained with hematoxylin.

Product Discontinued

HSPA8/HSC71/Hsc70 Antibody (SR39-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human HSPA8/HSC71/Hsc70 aa 597-646 / 646. (SwissProt: P11142 Human; SwissProt: P63017 Mouse; SwissProt: P63018 Rat)
• Localization: Cell membrane, Cytoplasm, Membrane, Nucleus, Spliceosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HSPA8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HSPA8/HSC71/Hsc70 Antibody (SR39-04)

• Heat shock 70 kDa protein 8
• heat shock 70kD protein 8
• heat shock 70kDa protein 8
• heat shock cognate 71 kDa protein
• heat shock cognate protein 54
• heat shock cognate protein, 71-kDa
• HSC54
• HSC70
• HSC70heat shock 70kd protein 10
• HSC71
• HSP71
• HSP73
• HSP73constitutive heat shock protein 70
• HSPA10
• HSPA8
• LAP1
• lipopolysaccharide-associated protein 1
• LPS-associated protein 1
• MGC131511
• MGC29929
• NIP71
• N-myristoyltransferase inhibitor protein 71

Background

Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPA8