Human B Cell Isolation Kit

Flow Cytometry: Human B Cell Isolation Kit [MAGH103-NOV] - Isolation of human CD19+ B cells from PBMC and whole blood using MagCellect Human B Cell Isolation kit. Human PBMC (top) and whole blood (bottom) were prepared as described in the Cell Preparation section. Cells were stained with Mouse Anti-Human CD19-PE Monoclonal Antibody and Mouse Anti-Human CD14-APC Monoclonal Antibody, either before (left) or after (right) isolation of B cells.

Product Discontinued

Human B Cell Isolation Kit Summary

• Description: The MagCellect Human B Cell Isolation Kit is designed to isolate B cells via a negative selection principle. The resulting cell preparation is highly enriched with B cells. Typical recovery of the targeted cell population ranges from 45-65% and the purity of recovered B cells ranges from 90-98%.
• Kit Type: Isolation Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

B cells are a subset of lymphocytes that express immunoglobulins on their cell surface. The B cell population is a vital component of the humoral immune response. B cell activation leads to antigen uptake and presentation, affinity maturation and class switching of the immunoglobulins, and antibody secretion. B cells represent a small portion (~5%) of the peripheral lymphocyte population making it difficult to obtain sufficient B cell quantities for many research applications. The ability to isolate and expand B cell populations ex vivo is valuable to researchers who require B cells for downstream applications including high throughput B cell assays and ex vivo differentiation of T cells. MagCellect products are designed for the isolation of cells in a "liquid phase". MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.