Human CD25/IL-2R alpha Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CD25/IL-2R alpha Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-2 sR alpha Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-2 sR alpha in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IL-2 sR alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2... sR alpha showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 sR alpha.
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• Specificity: Natural and recombinant human IL-2 sR alpha
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL2RA

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD25/IL-2R alpha Quantikine ELISA Kit

• CD25 antigen
• CD25
• IDDM10
• IL-2 R alpha
• IL-2 receptor subunit alpha
• IL2R alpha
• IL-2R subunit alpha
• IL2R
• IL2RA
• IL-2Ra
• IL-2-RA
• IL2-RA
• interleukin 2 receptor, alpha
• interleukin-2 receptor subunit alpha
• p55
• TAC antigen
• TCGFR

Background

CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2 R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2 R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CD25/IL-2R alpha (DR2A00)(36)

Publications using DR2A00

• K Ernzen, C Melvin, L Yu, C Phelps, S Niewiesk, PL Green, AR Panfil The PRMT5 inhibitor EPZ015666 is effective against HTLV-1-transformed T-cell lines in vitro and in vivo Frontiers in Microbiology, 2023-02-02;14(0):1101544. 2023-02-02 [PMID: 36819050] (Xenograft)
• M Trujillo-R, E Muñoz-Muel, A Serna-Gall, JM Praena-Fer, A Pérez-Góme, C Gasca-Capo, J Vitallé, J Peraire, ZR Palacios-B, JJ Cabrera, E Ruiz-Mateo, E Poveda, LE López-Cort, A Rull, A Gutierrez-, LF López-Cort Clinical, laboratory data and inflammatory biomarkers at baseline as early discharge predictors in hospitalized SARS-CoV-2 infected patients PLoS ONE, 2022-07-14;17(7):e0269875. 2022-07-14 [PMID: 35834501] (Human)
• DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang Cytokine response over the course of COVID-19 infection in pregnant women Cytokine, 2022-04-25;154(0):155894. 2022-04-25 [PMID: 35490452] (Human)
• S Buhelt, HB Søndergaar, MR Mahler, S Cobanovic, L Börnsen, C Ammitzbøll, AB Oturai, F Sellebjerg Biomarkers of systemic inflammation, soluble IL-2Ralpha and the multiple sclerosis-associated IL2RA SNP rs2104286 in healthy subjects and multiple sclerosis patients Multiple sclerosis and related disorders, 2021-07-07;54(0):103140. 2021-07-07 [PMID: 34304016] (Human)
• S Xu, H Li, P Qiao, G Xu, D Zhao, X Lin, Y Qin, H Yu, X Zhang, W Zhang, L Huang Neonatal hand, foot, and mouth disease due to coxsackievirus A6 in Shanghai BMC Pediatr, 2020-08-03;20(1):364. 2020-08-03 [PMID: 32741368] (Human)
• M Herrera, C Vera, Y Keynan, ZV Rueda Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review J Immunol Res, 2020-04-21;2020(0):8074183. 2020-04-21 [PMID: 32377537] (Human)
• T Gayden, FE Sepulveda, DA Khuong-Qua, J Pratt, ET Valera, A Garrigue, S Kelso, F Sicheri, LG Mikael, N Hamel, A Bajic, R Dali, S Deshmukh, D Dervovic, D Schramek, F Guerin, M Taipale, H Nikbakht, J Majewski, D Moshous, J Charlebois, S Abish, C Bole-Feyso, P Nitschke, B Bader-Meun, D Mitchell, C Thieblemon, M Battistell, S Gravel, VH Nguyen, R Conyers, JS Diana, C McCormack, HM Prince, M Besnard, S Blanche, PG Ekert, S Fraitag, WD Foulkes, A Fischer, B Neven, D Michonneau, G de Saint B, N Jabado Germline HAVCR2 mutations altering TIM-3 characterize subcutaneous panniculitis-like T cell lymphomas with hemophagocytic lymphohistiocytic syndrome Nat. Genet., 2018-10-29;0(0):. 2018-10-29 [PMID: 30374066] (Human)
• B Ribba, C Boetsch, TK Nayak, HP Grimm, JM Charo, S Evers, C Klein, JJL Tessier, JE Charoin, A Phipps, P Pisa, V Teichgräbe Prediction of the optimal dosing regimen using a mathematical model of tumour uptake for immunocytokine-based cancer immunotherapy Clin. Cancer Res., 2018-02-20;0(0):. 2018-02-20 [PMID: 29463551] (Human)
• LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa Reevaluation of immune activation in the era of cART and an aging HIV-infected population JCI Insight, 2017-10-19;2(20):. 2017-10-19 [PMID: 29046481] (Human)
• Incidence and Risk Factors for Developing Dengue-Associated Hemophagocytic Lymphohistiocytosis in Puerto Rico, 2008 - 2013 PLoS Negl Trop Dis, 2016-08-24;10(8):e0004939. 2016-08-24 [PMID: 27556807] (Human)
• Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions Cytokine, 2016-05-18;84(0):17-24. 2016-05-18 [PMID: 27208752] (Human)
• J Koreth, HT Kim, KT Jones, PB Lange, CG Reynolds, MJ Chammas, K Dusenbury, J Whangbo, S Nikiforow, EP Alyea, P Armand, CS Cutler, VT Ho, YB Chen, D Avigan, BR Blazar, JH Antin, J Ritz, RJ Soiffer Efficacy, durability, and response predictors of low-dose interleukin-2 therapy for chronic graft vs. host disease Blood, 2016-04-12;0(0):. 2016-04-12 [PMID: 27073224] (Human)
• Yu P, Petrus M, Ju W, Zhang M, Conlon K, Nakagawa M, Maeda M, Bamford R, Waldmann T Augmented efficacy with the combination of blockade of the Notch-1 pathway, bortezomib and romidepsin in a murine MT-1 adult T-cell leukemia model. Leukemia, 2014-08-14;29(3):556-66. 2014-08-14 [PMID: 25118879] (Human)
• Marzec M, Halasa K, Liu X, Wang H, Cheng M, Baldwin D, Tobias J, Schuster S, Woetmann A, Zhang Q, Turner S, Odum N, Wasik M Malignant transformation of CD4+ T lymphocytes mediated by oncogenic kinase NPM/ALK recapitulates IL-2-induced cell signaling and gene expression reprogramming. J Immunol, 2013-11-11;191(12):6200-7. 2013-11-11 [PMID: 24218456] (Human)
• Forsvoll J, Kristoffersen E, Oymar K Elevated levels of CXCL10 in the Periodic Fever, Aphthous stomatitis, Pharyngitis and cervical Adenitis syndrome (PFAPA) during and between febrile episodes; an indication of a persistent activation of the innate immune system. Pediatr Rheumatol Online J, 2013-10-17;11(1):38. 2013-10-17 [PMID: 24134207] (Human)
• Kassianos AJ, Hardy MY, Ju X, Vijayan D, Ding Y, Vulink AJ, McDonald KJ, Jongbloed SL, Wadley RB, Wells C, Hart DN, Radford KJ Human CD1c (BDCA-1)+ myeloid dendritic cells secrete IL-10 and display an immuno-regulatory phenotype and function in response to Escherichia coli. Eur. J. Immunol., 2012-06-01;42(6):1512-22. 2012-06-01 [PMID: 22678905] (Human)
• Singh R, Zhang Y, Pastan I, Kreitman RJ Synergistic antitumor activity of anti-CD25 recombinant immunotoxin LMB-2 with chemotherapy. Clin. Cancer Res., 2011-11-08;18(1):152-60. 2011-11-08 [PMID: 22068660] (Human)
• Ravandi F, O'Brien S, Jorgensen J, Pierce S, Faderl S, Ferrajoli A, Koller C, Challagundla P, York S, Brandt M, Luthra R, Burger J, Thomas D, Keating M, Kantarjian H Phase 2 study of cladribine followed by rituximab in patients with hairy cell leukemia. Blood, 2011-08-05;118(14):3818-23. 2011-08-05 [PMID: 21821712] (Human)
• Savan R, McFarland AP, Reynolds DA A novel role for IL-22R1 as a driver of inflammation. Blood, 2010-10-22;117(2):575-84. 2010-10-22 [PMID: 20971950] (Mouse)
• Schmidt H, Brown J, Mouritzen U, Selby P, Fode K, Svane IM, Cook GP, Mollerup DH, Geertsen PF Safety and clinical effect of subcutaneous human interleukin-21 in patients with metastatic melanoma or renal cell carcinoma: a phase I trial. Clin. Cancer Res., 2010-10-19;16(21):5312-9. 2010-10-19 [PMID: 20959407] (Human)
• Bachert C, Zhang N, Holtappels G, De Lobel L, Van Cauwenberge P, Liu S, Lin P, Bousquet J, Van Steen K Presence of IL-5 protein and IgE antibodies to staphylococcal enterotoxins in nasal polyps is associated with comorbid asthma. J. Allergy Clin. Immunol., 2010-11-01;126(5):962. 2010-11-01 [PMID: 20810157] (Human)
• Ishii T, Ishida T, Utsunomiya A Defucosylated humanized anti-CCR4 monoclonal antibody KW-0761 as a novel immunotherapeutic agent for adult T-cell leukemia/lymphoma. Clin. Cancer Res., 2010-02-16;16(5):1520-31. 2010-02-16 [PMID: 20160057] (Mouse)
• Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria. Transplantation, 2009-11-15;88(9):1109-16. 2009-11-15 [PMID: 19898207] (Human)
• Chen J, Zhang M, Ju W, Waldmann TA Effective treatment of a murine model of adult T-cell leukemia using depsipeptide and its combination with unmodified daclizumab directed toward CD25. Blood, 2008-10-23;113(6):1287-93. 2008-10-23 [PMID: 18948574] (Mouse)
• Kabagambe EK, Ordovas JM, Tsai MY, Borecki IB, Hopkins PN, Glasser SP, Arnett DK Smoking, inflammatory patterns and postprandial hypertriglyceridemia. Atherosclerosis, 2008-08-13;203(2):633-9. 2008-08-13 [PMID: 18804210] (Human)
• Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy. AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):886-90. 2007-07-01 [PMID: 17678471] (Human)
• Davis ID, Skrumsager BK, Cebon J, Nicholaou T, Barlow JW, Moller NP, Skak K, Lundsgaard D, Frederiksen KS, Thygesen P, McArthur GA An open-label, two-arm, phase I trial of recombinant human interleukin-21 in patients with metastatic melanoma. Clin. Cancer Res., 2007-06-15;13(12):3630-6. 2007-06-15 [PMID: 17575227] (Human)
• Cauley JA, Danielson ME, Boudreau RM, Forrest KY, Zmuda JM, Pahor M, Tylavsky FA, Cummings SR, Harris TB, Newman AB Inflammatory markers and incident fracture risk in older men and women: the Health Aging and Body Composition Study. J. Bone Miner. Res., 2007-07-01;22(7):1088-95. 2007-07-01 [PMID: 17419681] (Human)
• Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Blood, 2007-04-01;109(7):2806-14. 2007-04-01 [PMID: 17179229] (Human)
• Zhang Z, Zhang M, Garmestani K, Talanov VS, Plascjak PS, Beck B, Goldman C, Brechbiel MW, Waldmann TA Effective treatment of a murine model of adult T-cell leukemia using 211At-7G7/B6 and its combination with unmodified anti-Tac (daclizumab) directed toward CD25. Blood, 2006-03-28;108(3):1007-12. 2006-03-28 [PMID: 16569769] (Human)
• Zhang M, Yao Z, Zhang Z, Garmestani K, Goldman CK, Ravetch JV, Janik J, Brechbiel MW, Waldmann TA Effective therapy for a murine model of human anaplastic large-cell lymphoma with the anti-CD30 monoclonal antibody, HeFi-1, does not require activating Fc receptors. Blood, 2006-03-21;108(2):705-10. 2006-03-21 [PMID: 16551968] (Human)
• Zhang M, Zhang Z, Garmestani K, Goldman CK, Ravetch JV, Brechbiel MW, Carrasquillo JA, Waldmann TA Activating Fc receptors are required for antitumor efficacy of the antibodies directed toward CD25 in a murine model of adult t-cell leukemia. Cancer Res., 2004-08-15;64(16):5825-9. 2004-08-15 [PMID: 15313926] (Human)
• Xiao T, Fujita H, Saeki H, Mitsui H, Sugaya M, Tada Y, Kakinuma T, Torii H, Nakamura K, Asahina A, Tamaki K Thymus and activation-regulated chemokine (TARC/CCL17) produced by mouse epidermal Langerhans cells is upregulated by TNF-alpha and IL-4 and downregulated by IFN-gamma. Cytokine, 2003-08-01;23(4):126-32. 2003-08-01 [PMID: 12967648] (Human)
• Sedlaczek P, Frydecka I, Gabrys M, Van Dalen A, Einarsson R, Harlozinska A Comparative analysis of CA125, tissue polypeptide specific antigen, and soluble interleukin-2 receptor alpha levels in sera, cyst, and ascitic fluids from patients with ovarian carcinoma. Cancer, 2002-11-01;95(9):1886-93. 2002-11-01 [PMID: 12404282] (Human)
• Attanasio R, Brasky KM, Robbins SH, Jayashankar L, Nash RJ, Butler TM Age-related autoantibody production in a nonhuman primate model. Clin. Exp. Immunol., 2001-03-01;123(3):361-5. 2001-03-01 [PMID: 11298120] (Primate - Papio cynocephalus (Yellow Baboon))
• von Kobyletzki G, Pieck C, Hoffmann K, Freitag M, Altmeyer P Medium-dose UVA1 cold-light phototherapy in the treatment of severe atopic dermatitis. J. Am. Acad. Dermatol., 1999-12-01;41(6):931-7. 1999-12-01 [PMID: 10570376] (Human)

Bioinformatics

• Gene Symbol: IL2RA