Human CD8+ T Cell Isolation Kit

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Flow Cytometry: Human CD8+ T Cell Isolation Kit [MAGH112-NOV] - Ficolled human PBMCs before (A) and after (B) isolation of CD8+ T cells using the MagCellect Human CD8+ T Cell Isolation Kit. Dot plots reflect ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      MAGH112-NOV
    • Availability
      Product Discontinued

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Human CD8+ T Cell Isolation Kit Summary

Description
The MagCellect Human CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells via a negative selection principle. The resulting cell preparation is highly enriched with CD8+ T cells. Typical purity of recovered CD8+ T cells ranges from 75-87%.

Kit Type
Isolation Kit

Applications/Dilutions

Dilutions
  • Flow Cytometry

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. 1.0 ml of a Human CD8+ T Cell Biotinylated Antibody Cocktail
  2. 10 ml of a 10X Buffer
  3. 2.0 ml of a Streptavidin Ferrofluid

Background

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. MagCellect products are designed for the isolation of cells in a "liquid phase". MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) and behave like colloidal particles. This feature allows the ferrofluids to remain in solution without the need for mixing and additionally allows for efficient diffusion kinetics during the binding reaction. The proprietary manufacturing technology of MagCellect Ferrofluids generates particles with higher ligand binding capacity per mass compared to many other larger diameter magnetic particles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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