Human Flt-3 Ligand/FLT3L Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Flt-3 Ligand/FLT3L Quantikine ELISA Kit Summary

• Background: The Quantikine Human Flt-3/Flk-2 Ligand Immunoassay is a 4.0 or 4.5 hour solid phase ELISA designed to measure soluble human Flt-3 Ligand in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Flt-3 Ligand and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Flt-3 Ligand accurately. Results obtaine...d measuring natural human Flt-3 Ligand showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Flt-3 Ligand.
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• Specificity: Natural and recombinant human Flt-3 Ligand
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FLT3LG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Flt-3 Ligand/FLT3L Quantikine ELISA Kit

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Flt-3 Ligand/FLT3L (DFK00)(21)

Publications using DFK00

• KM Detjen, R Otto, Y Giesecke, L Geisler, P Riemer, H Jann, C Grötzinger, C Sers, A Pascher, T Lüdde, U Leser, B Wiedenmann, M Sigal, F Tacke, C Roderburg, L Hammerich Elevated Flt3L Predicts Long-Term Survival in Patients with High-Grade Gastroenteropancreatic Neuroendocrine Neoplasms Cancers, 2021-09-04;13(17):. 2021-09-04 [PMID: 34503273] (Human)
• JR Loughland, T Woodberry, D Oyong, KA Piera, FH Amante, BE Barber, MJ Grigg, T William, CR Engwerda, NM Anstey, JS McCarthy, MJ Boyle, G Minigo Reduced circulating dendritic cells in acute Plasmodium knowlesi and Plasmodium falciparum malaria despite elevated plasma Flt3 ligand levels Malaria Journal, 2021-02-16;20(1):97. 2021-02-16 [PMID: 33593383] (Human)
• S Saevarsdot, TA Olafsdotti, EV Ivarsdotti, GH Halldorsso, K Gunnarsdot, A Sigurdsson, A Johannesso, JK Sigurdsson, T Juliusdott, SH Lund, AO Arnthorsso, EL Styrmisdot, J Gudmundsso, GM Grondal, K Steinsson, L Alfredsson, J Askling, R Benediktss, R Bjarnason, AJ Geirsson, B Gudbjornss, H Gudjonsson, H Hjaltason, AB Hreidarsso, L Klareskog, I Kockum, H Kristjansd, TJ Love, BR Ludviksson, T Olsson, PT Onundarson, KB Orvar, L Padyukov, B Sigurgeirs, V Tragante, K Bjarnadott, T Rafnar, G Masson, P Sulem, DF Gudbjartss, P Melsted, G Thorleifss, GL Norddahl, U Thorsteins, I Jonsdottir, K Stefansson FLT3 stop mutation increases FLT3 ligand level and risk of autoimmune thyroid disease Nature, 2020-06-24;0(0):. 2020-06-24 [PMID: 32581359] (Human)
• X Yuan, X Qin, D Wang, Z Zhang, X Tang, X Gao, W Chen, L Sun Mesenchymal stem cell therapy induces FLT3L and CD1c+ dendritic cells in systemic lupus erythematosus patients Nat Commun, 2019-06-07;10(1):2498. 2019-06-07 [PMID: 31175312] (Human)
• Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance. Stem Cells, 2015-06-23;33(9):2838-49. 2015-06-23 [PMID: 26033476] (Human)
• Xiong W, Candolfi M, Liu C Human Flt3L generates dendritic cells from canine peripheral blood precursors: implications for a dog glioma clinical trial. PLoS ONE, 2010-06-11;5(6):e11074. 2010-06-11 [PMID: 20552015] (Human)
• Hatfield K, Ryningen A, Corbascio M, Bruserud O Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Int. J. Cancer, 2006-11-15;119(10):2313-21. 2006-11-15 [PMID: 16921487] (Human)
• T Kawase, T Nakazawa, T Eguchi, H Tsuzuki, Y Ueno, Y Amano, T Suzuki, M Mori, T Yoshida Effect of Fms-like tyrosine kinase 3 (FLT3) ligand (FL) on antitumor activity of gilteritinib, a FLT3 inhibitor, in mice xenografted with FL-overexpressing cells Oncotarget, 2019-10-22;10(58):6111-6123. 2019-10-22 [PMID: 31692922] (Human)
• Y Yuan, C Zhou, Q Yang, S Ma, X Wang, X Guo, Y Ding, J Tang, Y Zeng, D Li HIV-1 Tat protein inhibits the hematopoietic support function of human bone marrow mesenchymal stem cells Virus Res., 2019-09-12;273(0):197756. 2019-09-12 [PMID: 31521762] (Human)
• E Kalanxhi, S Meltzer, JV Schou, FO Larsen, S Dueland, K Flatmark, BV Jensen, KH Hole, T Seierstad, KR Redalen, DL Nielsen, AH Ree Systemic immune response induced by oxaliplatin-based neoadjuvant therapy favours survival without metastatic progression in high-risk rectal cancer Br. J. Cancer, 2018-04-26;118(10):1322-1328. 2018-04-26 [PMID: 29695770] (Human)
• AK Batsali, C Pontikoglo, D Koutroulak, KI Pavlaki, A Damianaki, I Mavroudi, K Alpantaki, E Kouvidi, G Kontakis, HA Papadaki Differential expression of cell cycle and WNT pathway-related genes accounts for differences in the growth and differentiation potential of Wharton's jelly and bone marrow-derived mesenchymal stem cells Stem Cell Res Ther, 2017-04-26;8(1):102. 2017-04-26 [PMID: 28446235] (Human)
• S Ciullini M, M Vignoli, G Colarusso, F Tucci, M Veltroni, S Frenos, V Tintori, M Aricò, V Bigley, M Collin, C Favre, E Gambineri Timely follow-up of a GATA2 deficiency patient allows successful treatment J. Allergy Clin. Immunol, 2016-07-29;138(5):1480-1483.e4. 2016-07-29 [PMID: 27481672] (Human)
• Reeves RK, Wei Q, Stallworth J, Fultz PN Systemic dendritic cell mobilization associated with administration of FLT3 ligand to SIV- and SHIV-infected macaques. AIDS Res. Hum. Retroviruses, 2009-12-01;25(12):1313-28. 2009-12-01 [PMID: 20001520] (Primate - Macaca nemestrina (Southern Pig-tailed Macaque))
• Cerebrospinal fluid Flt3 ligand level in patients with amyotrophic lateral sclerosis. Acta Neurol. Scand., 2006-09-01;114(3):205-9. 2006-09-01 [PMID: 16911350] (Human)
• Curtin JF, King GD, Barcia C, Liu C, Hubert FX, Guillonneau C, Josien R, Anegon I, Lowenstein PR, Castro MG Fms-like tyrosine kinase 3 ligand recruits plasmacytoid dendritic cells to the brain. J. Immunol., 2006-03-15;176(6):3566-77. 2006-03-15 [PMID: 16517725] (Human)
• Ali S, Curtin JF, Zirger JM, Xiong W, King GD, Barcia C, Liu C, Puntel M, Goverdhana S, Lowenstein PR, Castro MG Inflammatory and anti-glioma effects of an adenovirus expressing human soluble Fms-like tyrosine kinase 3 ligand (hsFlt3L): treatment with hsFlt3L inhibits intracranial glioma progression. Mol. Ther., 2004-12-01;10(6):1071-84. 2004-12-01 [PMID: 15564139] (Human)
• Drouet M, Mourcin F, Grenier N, Leroux V, Denis J, Mayol JF, Thullier P, Lataillade JJ, Herodin F Single administration of stem cell factor, FLT-3 ligand, megakaryocyte growth and development factor, and interleukin-3 in combination soon after irradiation prevents nonhuman primates from myelosuppression: long-term follow-up of hematopoiesis. Blood, 2003-10-02;103(3):878-85. 2003-10-02 [PMID: 14525791] (Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Briard D, Brouty-Boye D, Azzarone B, Jasmin C Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol., 2002-05-01;168(9):4326-32. 2002-05-01 [PMID: 11970974] (Human)
• Loeuillet C, Bernard G, Remy-Martin J, Saas P, Herve P, Douay L, Chalmers D Distinct hematopoietic support by two human stromal cell lines. Exp. Hematol., 2001-06-01;29(6):736-45. 2001-06-01 [PMID: 11378269] (Human)
• Solanilla A, Dechanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells. Blood, 2000-06-15;95(12):3758-64. 2000-06-15 [PMID: 10845907] (Human)

Bioinformatics

• Gene Symbol: FLT3LG