Human Lineage Marker/HLA-DR Antibody Pack

Product Discontinued

Human Lineage Marker/HLA-DR Antibody Pack Summary

• Specificity: This kit can also be used for the analysis of Myeloid Dendritic Cells.

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry
• Application Notes: Human LMAX (hLMAX) contains FITC-conjugated antibodies that, when added with HLA-DR as an additional marker, can be used to identify, enrich and/or deplete T, B, NK, and precursor myeloid cells (such as monocytes). The reagent can be used at 10 ul per one million cells in 100 ul total staining volume (or 100 ul whole blood). Red blood cell lysis buffer is recommended for lysing RBCs. Human FITC Lineage Markers and PerCP-Cy5.5 HLA-DR monoclonal antibody mix has been validated in flow cytometric analysis of Plasmacytoid Dendritic Cells from: whole peripheral blood within 24h of collection fresh collected PBMCs frozen PBMCs Use in Flow cell surface reported in scientific literature (PMID 24081865)

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24081865)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Tris or phosphate buffered saline with 0.05% BSA
• Preservative: 0.05% Sodium Azide

Alternate Names for Human Lineage Marker/HLA-DR Antibody Pack

• HLA-DR Antibody Mix
• hLMAX
• Human LMAX (hLMAX)
• Human LMAX

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Publications for Human Lineage Marker/HLA-DR Antibody Pack (NBP2-29608)(2)

Publications using NBP2-29608

• Takechi H et al. Clinical and immunological implications of increase in CD208+ dendritic cells in tonsils of patients with immunoglobulin A nephropathy. Nephrol Dial Transplant 2013-12-01 [PMID: 24081865] (Flow-CS, Human)
• Sato D, Suzuki Y, Kano T et al. Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse therapy in IgA nephropathy patients. Nephrol Dial Transplant. 2012-03-01 [PMID: 21778277] (Flow Cytometry Control, Flow-CS)
  
  Details:
  Plasmacytoid Dendritic Cell (pDC)/TLR9 Kit (IMG-6630K) & Human Lineage Marker/HLA-DR Antibody Mix (hLMAX). Flow (cell surface & intracellular): Primary human tonsillar B cells, Fig 3B.