Human Myeloid Dendritic Cell Flow Cytometry Kit

Flow Cytometry: Human Myeloid Dendritic Cell Flow Cytometry Kit [FMC016-NOV] - Dot plots show PBMCs stained simultaneously with the indicated antibodies as described in the procedure. BDCA-1+/CD11c+, BDCA-3+/CD11c+, and CD16+/CD11c+ are the main myeloid dendritic cell populations present in human blood. Quadrants were set based on isotype controls.

Product Discontinued

Human Myeloid Dendritic Cell Flow Cytometry Kit Summary

• Description: This product is designed for the single-step staining of human myeloid dendritic cells (mDCs) using four conjugated antibodies and corresponding isotype controls.
• Kit Type: Flow Cytometry Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

Dendritic cells (DCs) are key mediators of both innate and adaptive immune responses. Immature DCs express specific pattern recognition receptors that serve as expression markers and allow for the capture and processing of foreign antigens following infection. Upon activation, immature dendritic cells mature and increase the expression of class II MHC and co-stimulatory molecules important for effective antigen presentation to naive T cells. Cytokines produced by DCs can also promote the differentiation of CD4+ T helper cells as part of immune activation. The ability to differentiate dendritic cell populations ex vivo is valuable to researchers who require either immature or mature dendritic cells for downstream applications including high throughput assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.