Human Regulatory T Cell Flow Cytometry Kit

Flow Cytometry: Human Regulatory T Cell Flow Cytometry Kit [FMC021-NOV] - Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A Mouse Anti-Human CD4 Fluorescein-conjugated Monoclonal Antibody and (B) Mouse Anti-Human IL-2Ra/CD25 APC-conjugated Monoclonal Antibody, followed by intracellular staining using Rabbit Anti-Human/ Mouse FoxP3 PE-conjugated Antigen Affinity-purified Monoclonal Antibody. To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit. Cells were gated on lymphocytes.

Product Discontinued

Human Regulatory T Cell Flow Cytometry Kit Summary

• Description: This product is designed for the flow cytometric analysis of human regulatory T cells using three fluorochrome-conjugated antibodies.
• Kit Type: Flow Cytometry Kit

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.

Background

The primary function of regulatory T cells, also known as suppressor T cells, is to maintain immune homeostasis. This involves suppression of successful immune responses and control of self versus non-self recognition. Failure of the latter results in autoimmune destruction of host cells and tissue. Like other T cells, regulatory T cells mature in the thymus where they are characterized by the variable expression of CD8, CD4, CD25 and FoxP3. The importance of FoxP3 is underlined by genetic mutations in this molecule which result in a fatal autoimmune disorder known as Immune dysregulation, Polyendocrinopathy, Enteropathy X-linked (IPEX) syndrome. Conversely, taking advantage of the immunosuppressive potential of regulatory T cells is an important area for advancement in the fields of autoimmune disease and organ transplantation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.