Human TNF-alpha Quantikine ELISA Kit

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Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Human TNF-alpha Quantikine ELISA Kit Summary

Background
The Quantikine Human TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure human TNF-alpha in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human TNF-alpha and antibodies raised against this protein. It has been shown to accurately quantitate the recombinant factor. Results obtained with naturally occurring TNF-alpha samples showe...d linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human TNF-alpha .
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Specificity
Natural and recombinant human TNF-alpha .
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DTA00D in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TNF-alpha Quantikine ELISA Kit

  • IL-10C
  • IL19
  • IL-19
  • interleukin 19
  • MDA1
  • melanoma differentiation associated protein-like protein
  • NG.1Melanoma differentiation-associated protein-like protein
  • ZMDA1interleukin-19

Background

Tumor Necrosis Factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11).  
Human TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (12, 13). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus monkey, and 71%-92% aa identity with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha . It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (14). Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI (15, 16). Shedding of membrane bound TNF-alpha by TACE/ ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (17-19).  
TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (20, 21) and the hematopoietic cell-restricted 78-80 kDa TNF RII (22, 23), both of which are also expressed as homotrimers (1, 24). Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NF kappa B activation (25-28). Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis (3, 29). Soluble forms of both types of receptors are released into human serum and urine and can neutralize the biological activity of TNF-alpha (30-32).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-19 (DTA00D)(548)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Primate, Primate - Cholrocebus pygerythrus (Vervet Monkey), Primate - Pan troglodytes (Chimpanzee), Rabbit.

We have publications tested in 3 applications: ELISA, ELISA (Standard), ELISA Capture.


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Human
(535)
Mouse
(7)
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Primate
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Primate - Cholrocebus pygerythrus (Vervet Monkey)
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Showing Publications 1 - 10 of 548. Show All 548 Publications.
Publications using DTA00D Applications Species
Liu, WC;Wu, MY;Lim, PS; Alterations in Autophagic Function and Endoplasmic Reticulum Stress Markers in the Peripheral Blood Mononuclear Cells of Patients on Hemodialysis International journal of molecular sciences 2025-01-07 [PMID: 39859163] (Human) Human
Soltani, M;Shahsavani, A;Hopke, PK;Bakhtiarvand, NA;Abtahi, M;Rahmatinia, M;Kermani, M; Investigating the inflammatory effect of microplastics in cigarette butts on peripheral blood mononuclear cells Scientific reports 2025-01-02 [PMID: 39747550] (Human) Human
Sarmin, M;Akter, F;Islam, AN;Mahfuz, M;Das, S;Sharifuzzaman, ;Hasan, SMT;Bhuiyan, TR;Rahman, M;Gazi, A;Matin, FB;Tariqujjaman, M;Shahrin, L;Islam, M;Mahmud, AM;Banu, S;Chisti, MJ;Qadri, F;Clemens, JD;Ahmed, T; Cytokine Storm among Bangladeshi adults with COVID-19: A prospective cohort study Heliyon 2024-12-15 [PMID: 39654713] (Human) Human
Kawasaki, R;Sakata, A;Tatsumi, K;Mitani, S;Takeda, M;Kasuda, S;Matsumoto, N;Harada, S;Soeda, T;Nishida, Y;Yoshimura, Y;Shima, M; ?-hydroxybutyrate suppresses pathological changes of blood-induced arthropathy in rats Scientific reports 2024-11-29 [PMID: 39614095] (Human) Human
Li, X;Yan, Y;Wang, Z;Hou, J;Meng, Y;Cui, D;Long, Y;Li, M;Yang, R; The link between osteoporosis and frozen shoulder: exploring the therapeutic effect of TAK715 on reversing fibrosis and protecting against osteoporosis via the p38 MAPK signaling pathway BMC musculoskeletal disorders 2024-11-21 [PMID: 39574076] (Human) Human
Yang, W-S;Kim, D;Kang, S;Lai, C-J;Cha, I;Chang, P-C;Jung, JU; Development of KSHV vaccine platforms and chimeric MHV68-K-K8.1 glycoprotein for evaluating the in vivo immunogenicity and efficacy of KSHV vaccine candidates mBio 2024-10-30 [PMID: 39475238] (Mouse) Mouse
Zhen, X;Kim, J;Kang, JS;Choi, BJ;Park, KH;Lee, DS;Hong, SH;Lee, JH; Homology-independent targeted insertion-mediated derivation of M1-biased macrophages harbouring Megf10 and CD3? from human pluripotent stem cells EBioMedicine 2024-10-08 [PMID: 39383607] (Human) Human
Guerrero, JA;Klysz, DD;Chen, Y;Malipatlolla, M;Lone, J;Fowler, C;Stuani, L;May, A;Bashti, M;Xu, P;Huang, J;Michael, B;Contrepois, K;Dhingra, S;Fisher, C;Svensson, KJ;Davis, KL;Kasowski, M;Feldman, SA;Sotillo, E;Mackall, CL; GLUT1 overexpression in CAR-T cells induces metabolic reprogramming and enhances potency Nature communications 2024-10-06 [PMID: 39370422] (Human) Human
Longo, M;Paolini, E;Di Benedetto, P;Tomassini, E;Meroni, M;Dongiovanni, P; DGAT1 and DGAT2 Inhibitors for Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) Management: Benefits for Their Single or Combined Application International journal of molecular sciences 2024-08-21 [PMID: 39201759] (Human) Human
Malek, E;Rana, PS;Swamydas, M;Daunov, M;Miyagi, M;Murphy, E;Ignatz-Hoover, JJ;Metheny, L;Kim, SJ;Driscoll, JJ; The TGF? type I receptor kinase inhibitor vactosertib in combination with pomalidomide in relapsed/refractory multiple myeloma: a phase 1b trial Nature communications 2024-08-27 [PMID: 39191755] (Human) Human
Show All 548 Publications.

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