Hydrogen Peroxide Detection Kit (Colorimetric)

Hydrogen Peroxide Colorimetric Assay Kit [KA1017] - drogen Peroxide [KA1017] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Product Discontinued

Hydrogen Peroxide Detection Kit (Colorimetric) Summary

• Description: Quality control test: Standard curve Hydrogen Peroxide Colorimetric Detection Kit is a higher throughput assay for the quantitative measurement of hydrogen peroxide in culture supernatants, serum, plasma, and urine from any species.
• Standard Curve Range: 106.25 to 3400 ng/mL
• Sensitivity: 51.25 ng/mL
• Assay Type: Colorimetric
• Inter-Assay: 3.20%
• Intra-Assay: 5.66%
• Spike Recovery: 96.35%
• Sample Volume: 50 uL
• Kit Type: Detection Kit (Colorimetric)

Applications/Dilutions

• Dilutions:
  • Quantification

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Background

Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states. Functioning through NF kappa-B and other factors, hydroperoxide-mediated pathways have been linked to asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down's Syndrome. Measurement of this reactive species helps to determine how oxidative stress modulates varied intracellular pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Hydrogen Peroxide Kit (KA1017)(5)

Publications using KA1017

• F Qin, X Liu, J Chen, S Huang, W Wei, Y Zou, X Liu, K Deng, S Mo, J Chen, X Chen, Y Huang, W Liang Anti-TGF-&beta attenuates tumor growth via polarization of tumor associated neutrophils towards an anti-tumor phenotype in colorectal cancer J Cancer, 2020-02-14;11(9):2580-2592. 2020-02-14 [PMID: 32201528]
• Mishalian I, Bayuh R, Levy L et al. Tumor-associated neutrophils (TAN) develop pro-tumorigenic properties during tumor progression. Cancer Immunol Immunother. 2013-10-04 [PMID: 24092389]
• Okuda M, Inoue N, Azumi H et al. Expression of glutaredoxin in human coronary arteries: its potential role in antioxidant protection against atherosclerosis. Arterioscler Thromb Vasc Biol. 2001-09-01 [PMID: 11557676]
• Peiro C, Lafuente N, Matesanz N et al. High glucose induces cell death of cultured human aortic smooth muscle cells through the formation of hydrogen peroxide. Br J Pharmacol. 2001-08-01 [PMID: 11487505]
• Uesugi M, Yoshida K, Jasin HE. Inflammatory properties of IgG modified by oxygen radicals and peroxynitrite. J Immunol. 2000-12-01 [PMID: 11086095]