Hydroxyproline Assay Kit (Colorimetric)

Hydroxyproline Colorimetric Assay Kit [NBP2-59747]

Hydroxyproline Colorimetric Assay Kit [NBP2-59747]

Product Discontinued

Hydroxyproline Assay Kit (Colorimetric) Summary

• Description: This kit uses an acidic developer solution to accurately measure hydroxyproline in tissue and protein/peptidase hydrolysates without the use of toxic perchlorates. Featuring a simple and quick protocol, this hydroxyproline assay yields a brightly-colored chromophore with an absorbance peak at 560 nm. The assay can detect a minimum of 0.1 ug hydroxyproline per well in a 96-well format and generates colorimetric results that are virtually identical to the classical perchloric acid-based method.
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab222941)
• Kit Type: Assay Kit (Colorimetric)

Applications/Dilutions

Publications

Read Publications using NBP2-59747.

Packaging, Storage & Formulations

• Storage: Store at -20 °C.

Background

Hydroxyproline ((2S,4R)-4-hydroxyproline, Hyp) is a nonproteinogenic amino acid formed by the enzyme prolyl-4-hydroxylase, which catalizes the post-translational modification of polypeptide proline residues following protein synthesis. In animals, hydroxyproline is found almost entirely in collagen, which contains approximately 12-14% hydroxyproline by mass (the fibrilous protein elastin also contains small quantities of hydroxyproline). Hydroxyproline in tissue hydrolysates is thus a direct measure of the amount of collagen present. Collagen is the most abundant protein in mammals and many diseases are believed to affect collagen turnover, including tumor invasion and metastasis, rheumatoid arthritis, cardiopulmonary fibrosis and muscular dystrophy. The classical assay for determination of Hyp is based upon oxidation of Hyp to a pyrrole intermediate, followed by reaction with Ehrlich's reagent dissolved in concentrated perchloric acid (HClO4). Perchloric acid is a hazardous material that is both toxic and highly reactive, requiring special handling and waste-disposal protocols.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Hydroxyproline Kit (NBP2-59747)(7)

Publications using NBP2-59747

• Bai L, Wang J, Wang X et al. Combined therapy with pirfenidone and nintedanib counteracts fibrotic silicosis in mice AU 2023-01-01 (ELISA)
• Bai L, Wang J, Wang X et Al. Combined therapy with pirfenidone and nintedanib counteracts fibrotic silicosis in mice Br J Pharmacol 2024-11-15 [PMID: 39546810]
• Song M, Wang J, Sun Y Et al. Inhibition of gasdermin D-dependent pyroptosis attenuates the progression of silica-induced pulmonary inflammation and fibrosis Acta Pharm Sin B 2022-05-09 [PMID: 35530143]
• Yang S, Liu P, Gao T et al. Every road leads to Rome: therapeutic effect and mechanism of the extracellular vesicles of human embryonic stem cell-derived immune and matrix regulatory cells administered to mouse models of pulmonary fibrosis through different routes Stem cell research & therapy 2022-04-12 [PMID: 35413874]
• Song MY, Wang JX, Sun YL Et al. Tetrandrine alleviates silicosis by inhibiting canonical and non-canonical NLRP3 inflammasome activation in lung macrophages Acta pharmacologica Sinica 2021-08-20 [PMID: 34417574]
• Cao Zj, Liu Y, Zhang Z Et Al. Pirfenidone ameliorates silica-induced lung inflammation and fibrosis in mice by inhibiting the secretion of interleukin-17A Acta pharmacologica Sinica 2021-07-27 [PMID: 34316030]
• Cao Z, Song M, Liu Y et al. A novel pathophysiological classification of silicosis models provides some new insights into the progression of the disease Ecotoxicology and Environmental Safety 2020-10-01 [PMID: 32622305]