Recombinant Human IFN-alpha 2 Protein

SDS-Page: Recombinant Human Interferon alpha 2b Protein [NBP2-34984]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IFN-alpha 2 Protein Summary

• Description: A single polypeptide chain containing 165 amino acids corresponding to IFN-alpha 2 Source: Yeast

  Uniprot ID: P01563

  Amino Acid Sequence: CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

• Details of Functionality: Interferon alpha 2b Protein is fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.6 x 10^8 IU/mg.
• Source: Yeast
• Protein/Peptide Type: Recombinant Protein
• Gene: IFNA2
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of IFN-alpha 2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 19.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4, with 0.02 % Tween-20.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 98 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IFN-alpha 2 Protein

• alpha-2a interferon
• Ifa2
• IFNA
• IFNA2
• IFNA2a
• IFNA2b
• IFNA2c
• IFNAA
• IFNalpha 2
• IFN-alpha 2
• IFN-alpha-2
• IFN-alphaA
• INFA2
• interferon alpha 2b
• interferon alpha A
• interferon alpha-2
• Interferon alpha-A
• interferon, alpha 2
• LeIF A
• MGC125764
• MGC125765

Background

IFN-alphas are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-alpha family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IFNA2
• Uniprot:
  • P01563()
  • P01563()
  • P01563()