IFN-alpha Antibody (MMHA-11) [Unconjugated]

• Reactivity: Hu
• Applications: ELISA, B/N
• Clone: MMHA-11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IFN-alpha Antibody (MMHA-11) [Unconjugated] Summary

• Immunogen: Human Leukocyte Interferon
• Specificity: One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN- alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol . 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN- alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNA2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <1.0 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Neutralization

Packaging, Storage & Formulations

• Storage: After receipt, the product may be stored at -20°C for short-term use ( ≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
• Buffer: Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
• Preservative: No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-alpha Antibody (MMHA-11) [Unconjugated]

• IFNA
• IFNA2
• IFNA2b
• IFNalpha
• IFN-alpha
• IFN-alphaA
• INFA2
• interferon alpha 2
• LeIF D

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-alpha Antibody (21112-1)(3)

Publications using 21112-1

• L Bin, X Li, B Richers, JE Streib, JW Hu, P Taylor, DYM Leung ANKRD1 regulates innate immune responses against herpes simplex virus 1: A potential role in eczema herpeticum J. Allergy Clin. Immunol., 2018-01-31;0(0):. 1/31/2018 [PMID: 29371118]
• Richez C, Yasuda K, Watkins AA TLR4 ligands induce IFN-alpha production by mouse conventional dendritic cells and human monocytes after IFN-beta priming. J. Immunol., 2009-01-15;182(2):820-8. 2009-01-15 [PMID: 19124725] (ELISA Development, Mouse)
• Lee PY, Li Y, Richards HB, Chan FS, Zhuang H, Narain S, Butfiloski EJ, Sobel ES, Reeves WH, Segal MS Type I interferon as a novel risk factor for endothelial progenitor cell depletion and endothelial dysfunction in systemic lupus erythematosus. Arthritis Rheum., 2007-11-01;56(11):3759-69. 2007-11-01 [PMID: 17968925] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IFNA2