IFN-alpha/beta R1 Antibody (A5-A3)

Western Blot: IFN-alpha/beta R1 Antibody (A5-A3) [NBP2-75534] - Analysis of IFNAR1 on different lysates using anti- IFNAR1 antibody at 1/500 dilution. Positive control: Lane 1: K562 Lane 1: A431 Lane 1: Siha Lane 1: Mouse brain tissue.

Immunocytochemistry/Immunofluorescence: IFN-alpha/beta R1 Antibody (A5-A3) [NBP2-75534] - Staining IFNAR1(green) in SHG-44 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (A5-A3) [NBP2-75534] - Analysis of paraffin-embedded human skin tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: IFN-alpha/beta R1 Antibody (A5-A3) [NBP2-75534] - Staining IFNAR1(red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (A5-A3) [NBP2-75534] - Analysis of paraffin-embedded human lung tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: IFN-alpha/beta R1 Antibody (A5-A3) [NBP2-75534] - Analysis of paraffin-embedded human colon tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: A5-A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

IFN-alpha/beta R1 Antibody (A5-A3) Summary

• Immunogen: Recombinant protein within human IFN-alpha/beta R1 aa 28-127 (Extracellular). (SwissProt: P17181 Human; SwissProt: P33896 Mouse)
• Localization: Endosome. Cell membrane.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFNAR1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein G purified

Alternate Names for IFN-alpha/beta R1 Antibody (A5-A3)

• alpha-type antiviral protein
• AVP
• beta-type antiviral protein
• CRF2-1
• Cytokine receptor class-II member 1
• Cytokine receptor family 2 member 1
• human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC
• IFN-alpha/beta R1
• IFN-alpha/beta receptor 1
• IFN-alpha-REC
• IFNAR
• IFNAR1
• IFN-aR1
• IFNBR
• IFNbR1
• IFN-bR1
• IFN-R-1
• interferon (alpha, beta and omega) receptor 1
• interferon alpha/beta receptor 1
• interferon-alpha/beta receptor alpha chain
• interferon-beta receptor 1
• Type I interferon receptor 1

Background

Interferon alpha receptor 1 (IFN-alpha R1) is a class II cytokine receptor which belongs to type I human interferons (IFNs) family. IFNs plays a role in antiviral, antiproliferative, immunomodulatory, antitumor, and antiparasitic activities by inducing transcription of IFN-stimuated genes (ISGs) through activation of the Jak-STAT pathway. Specifically, IFN- R1 is a 135-kda signal transducing subunit of the IFN alpha/beta receptor complex (IFN-alpha R) (1). IFN-alpha R1 is phospholyated through PTK- and PKC-mediated phosphorylation, which initiates docking of STAT proteins to IFNs. This leads to activation of STAT proteins by PTK-mediated phosphorylation. The mechanism behind the amplification of IFN-alpha R complex mediated transmembrane signaling has been linked to tyrosine phosphorylation on IFN-alpha R1 (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFN-alpha/beta R1 Antibody (NBP2-75534)(1)

Publication using NBP2-75534

• Jana A, Wang X, Leasure JW et al. Increased Type I interferon signaling and brain endothelial barrier dysfunction in an experimental model of Alzheimer's disease Scientific reports 2022-10-01 [PMID: 36182964] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: IFNAR1