IFN-gamma Antibody [Biotin]

• Applications: WB, ELISA(Det), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IFN-gamma Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human IFN-gamma (R&D Systems, Catalog # 285-IF)
  Met1-Gln144
  Accession # Q14609
• Specificity: Detects human IFN-gamma in ELISAs and Western blots. In sandwich immunoassays, less than 3% cross-reactivity with recombinant rhesus macaque IFN‑ gamma is observed and less than 0.05% cross-reactivity with recombinat rat IFN-gamma , recombinant feline IFN-gamma , recombinant equine IFN-gamma , recombinant cotton rat IFN-gamma , recombinant canine IFN-gamma , recombinant porcine IFN-gamma , and recombinant mouse IFN-gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFNG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Application Notes: ELISA Capture: Human IFN-gamma Antibody (Catalog # MAB2852)
  ELISA Detection: Human IFN-gamma Biotinylated Antibody (Catalog # BAF285)
  Standard: Recombinant Human IFN-gamma (Catalog # 285-IF)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IFN-gamma Antibody (BAF285). (Showing 1 - 1 of 1 FAQ).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG