Recombinant Human IFN-gamma Protein

Bioactivity: Recombinant Human IFN-gamma Protein [NBP2-51820] - Human IFN-gamma stimulates cytotoxicity of the WiDr human colon colorectal adenocarcinoma cells. The ED50 range is less than 0.5ng/ml.

SDS-Page: Recombinant Human IFN-gamma Protein [NBP2-51820] - 15% SDS Page (3 ug)

Product Discontinued

Recombinant Human IFN-gamma Protein Summary

• Description: An un-tagged bioactive recombinant protein corresponding to the amino acids 24-161 of Human IFN-gamma
  
  

  Source: E.coli

  Amino Acid Sequence: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG

• Details of Functionality: Human IFN-gamma stimulates cytotoxicity of the WiDr human colon colorectal adenocarcinoma cells. The ED50 range is less than 0.5ng/ml.
• Source: E. coli
• Protein/Peptide Type: Biologically Active Protein
• Gene: IFNG
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 16.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human IFN-gamma Protein

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

FAQs for IFN-gamma Biologically Active Protein (NBP2-51820). (Showing 1 - 1 of 1 FAQ).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG