Human IFN-gamma ELISA Kit (Colorimetric)

ELISA: IFN-gamma ELISA Kit [NBP1-91174] - Standard curve

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
• Standard Curve Range: 1.6 - 100 pg/mL
• Sensitivity: 0.99 pg/mL

Human IFN-gamma ELISA Kit (Colorimetric) Summary

• Description: The human IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-gamma.
• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human IFN gamma positive serum. There was no crossreactivity detected.
• Standard Curve Range: 1.6 - 100 pg/mL
• Sensitivity: 0.99 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.70%
• Intra-Assay: CV% < 4.50%
• Spike Recovery: The recovery ranged from 88% to 112% with an overall mean recovery of 97%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IFNG

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IFN-gamma ELISA Kit (Colorimetric)

• IFG
• IFI
• IFNG
• IFNgamma
• IFN-gamma
• Immune interferon
• interferon gamma
• interferon, gamma

Background

IFN-gamma, also called Type II interferon, is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. The human IFN-gamma gene, situated on chromosome 12, contains three introns; the four exons code for a polypeptide of 166 amino acids, 20 of which constitute the signal peptide. In contrast to IFN-alpha and IFN-beta synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. IFN-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. DetailsDocumentationAdditional FormatsReferences

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for IFN-gamma ELISA Kit (NBP1-91174)(1)

Publication using NBP1-91174

• Cui R, Rekasi H, Hepner-Schefczyk M et al. Human mesenchymal stromal/stem cells acquire immunostimulatory capacity upon cross-talk with natural killer cells and might improve the NK cell function of immunocompromised patients Stem Cell Res Ther 2016-07-08 [PMID: 27388156] (ELISA, Human)

FAQs for IFN-gamma ELISA Kit (NBP1-91174). (Showing 1 - 1 of 1 FAQ).

1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
   • We are research use only.

Bioinformatics

• Gene Symbol: IFNG