Recombinant Human IGF-I/IGF-1 Protein

SDS-Page: Human IGF-I Protein [NBP2-35000]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human IGF-I/IGF-1 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 70 amino acids corresponding to IGF-I/IGF-1 Source: E. coli

  Uniprot ID: P05019

  Amino Acid Sequence: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

• Details of Functionality: IGF1 Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 x 10^5 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IGF1
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.01 EU/ug of IGF-I/IGF-1 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 7.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human IGF-I/IGF-1 Protein

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

Insulin-like Growth Factor I (IGF-I), also known as Somatomedin C, is the dominant effector of Growth Hormone (GH). IGF-I regulates glucose, fatty acid, and protein metabolism, steroid hormone activity, cartilage and bone metabolism, muscle regeneration, tumor progression, and cellular differentiation and migration. IGF-I effects are mediated primarily by IGF-I R, and its bioavailability is regulated by IGF binding proteins (IGFBPs). Mature human IGF-I shares about 95% aa sequence identity with the mouse and rat IGF-I. Long R3 IGF-I (LR3 IGF-I) is a synthetic analog of IGF-I that has a Glu3Arg substitution and a 13 aa N-terminal extension derived from methionyl porcine GH. It retains the ability to bind IGF-I R and Insulin R but shows reduced affinity for IGFBPs. As a result, LR3 IGF-I has an increased half-life and displays increased biological potency compared to IGF-I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IGF-I/IGF-1 Recombinant Protein (NBP2-35000)(2)

Publications using NBP2-35000

• Ravindran P, PUschel AW An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation Life science alliance 2023-03-01 [PMID: 36543541] (In vitro)
• Wei Li, Linguo Li, Kunlan Li et al. Expression of biologically active human insulin-like growth factor 1 in Arabidopsis thaliana seeds via oleosin fusion technology. Biotechnol Appl Biochem 2011-05-01 [PMID: 21679237]

Bioinformatics

• Gene Symbol: IGF1
• Uniprot:
  • P05019()
  • P05019()
  • P05019()