Recombinant Bovine IGF-I/IGF-1 Protein

ELISA: Recombinant Bovine IGF-I/IGF-1 Protein [NBP2-62426] - Bovine Insulin-Like Growth Factor I ELISARecombinant Bovine insulin-like growth factor Idetected using Goat anti Bovine insulin-like growth factor I as the capture reagent and biotinylated Goat anti Bovine insulin-like growth factor I as the detection reagent followed by Streptavidin:HRP.

• Reactivity: Bv
• Applications: WB, ELISA
• Concentration: LYOPH

Recombinant Bovine IGF-I/IGF-1 Protein Summary

• Description: Recombinant bovine insulin-like growth factor I.
  
  

  Source:Pichia pastoris

  Amino Acid Sequence:aa 50-119 of native bovine IGF-I, UniProt P07455

• Preparation Method: Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
• Source: Pichia pastoris
• Protein/Peptide Type: Recombinant Protein
• Gene: IGF1
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Theoretical MW: 7.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 10% Trehalose
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with 20 ul sterile PBS containing 0.1% carrier protein. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix vial after reconstitution.

Alternate Names for Recombinant Bovine IGF-I/IGF-1 Protein

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IGF1
• Uniprot:
  • P07455(Bovine)