IGF-I R/IGF1R Antibody [Unconjugated]

IGF-I R/IGF1R was detected in immersion fixed MCF-7 human breast cancer cell line (positive control, left panel) and HDLM-2 human Hodgkin's lymphoma cell line (negative control, right panel) using Goat Anti-Human/Mouse IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocols for Fluorescent ICC Staining of Cells on Coverslipsand Fluorescent ICC Staining of Non-adherent Cells.

IGF-I R/IGF1R was detected in immersion fixed frozen sections of mouse embryo using Goat Anti-Human IGF-I R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

IGF-I R/IGF1R was detected in immersion fixed paraffin-embedded sections of human placenta (chorionic villi) using 15 µg/mL Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Recombinant Human IGF‑I (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF‑I (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I R/IGF1R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-305-NA). The ND50 is typically 0.5‑1.5 µg/mL.

Representative H&E stained sections of hyperplasia (A) and normal mammary ducts (B) that developed following transplantation of pubertal MTB-IGFIR mammary epithelial cells that did not progress to palpable tumors.Immunostaining for IGF-IR (C, D) revealed high levels of expression with normal mammary ducts indicating successful engraftment of transgenic tissue. Scale bars, 200 µM (A–C) and 100 µM (D). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Representative H&E stained sections of hyperplasia (A) and normal mammary ducts (B) that developed following transplantation of pubertal MTB-IGFIR mammary epithelial cells that did not progress to palpable tumors.Immunostaining for IGF-IR (C, D) revealed high levels of expression with normal mammary ducts indicating successful engraftment of transgenic tissue. Scale bars, 200 µM (A–C) and 100 µM (D). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry for the IGF-IR (brown stain) in mammary epithelial cells of a 2 day old female mice captured at 100x (A) or 200x (B) magnification.Scale bars, 200 µM (A) and 100 µM (B). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0108781), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu, Mu
• Applications: WB, IHC, B/N, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IGF-I R/IGF1R Antibody [Unconjugated] Summary

• Immunogen: Recombinant Human IGF-I R/IGF1R extracellular domain
  Accession # P08069
• Specificity: Detects human and mouse IGF-I R/IGF1R in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25-50% cross-reactivity with recombinant mouse IGF-I R is observed. In direct ELISAs, less than 1% cross-reactivity with recombinant human IGF‑II R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGF1R
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 0.5-1.5 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I R/IGF1R Antibody [Unconjugated]

• CD221 antigen
• CD221
• EC 2.7.10
• EC 2.7.10.1
• IGF1R
• IGF-1R
• IGF-I R
• IGF-I receptor
• IGFIR
• IGF-IR
• IGFR
• insulin-like growth factor 1 receptor
• Insulin-like growth factor I receptor
• JTK13
• MGC142170
• MGC142172
• MGC18216
• soluble IGF1R variant 1
• soluble IGF1R variant 2

Background

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

1. Rechler, M.M. and S.P. Nissley (1990) in Insulin-Like Growth Factors. Sporn, M.B. and A.B. Roberts (eds): Peptide Growth Factors and Their Receptors I, New York: Springer-Verlag, p. 263.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-I R/IGF1R Antibody (AF-305-NA)(26)

Publications using AF-305-NA

• Yu, D;Jain, S;Wangzhou, A;De Florencio, S;Zhu, B;Kim, JY;Choi, JJ;Paredes, MF;Nowakowski, TJ;Huang, EJ;Piao, X; Microglia regulate GABAergic neurogenesis in prenatal human brain through IGF1 bioRxiv : the preprint server for biology 2024-10-20 [PMID: 39464051] (Immunohistochemistry, Human)
• Alyssa J. J. Paganoni, Rossella Cannarella, Roberto Oleari, Federica Amoruso, Renata Antal, Marco Ruzza et al. Insulin-like Growth Factor 1, Growth Hormone, and Anti-Müllerian Hormone Receptors Are Differentially Expressed during GnRH Neuron Development International Journal of Molecular Sciences 8/22/2023 [PMID: 37685880]
• Niklas Ortenlöf, Suvi Vallius, Helena Karlsson, Claes Ekström, Amanda Kristiansson, Bo Holmqvist et al. Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3 Fluids and Barriers of the CNS 8/15/2023 [PMID: 37582792]
• Niklas Ortenlöf, Suvi Vallius, Helena Karlsson, Claes Ekström, Amanda Kristiansson, Bo Holmqvist et al. Characterization of choroid plexus in the preterm rabbit pup following subcutaneous administration of recombinant human IGF-1/IGFBP-3 Fluids and Barriers of the CNS 2023-08-15 [PMID: 37582792] (Immunohistochemistry, Rabbit)
• Pan, L;Cho, KS;Wei, X;Xu, F;Lennikov, A;Hu, G;Tang, J;Guo, S;Chen, J;Kriukov, E;Kyle, R;Elzaridi, F;Jiang, S;Dromel, PA;Young, M;Baranov, P;Do, CW;Williams, RW;Chen, J;Lu, L;Chen, DF; IGFBPL1 is a master driver of microglia homeostasis and resolution of neuroinflammation in glaucoma and brain tauopathy Cell reports 2023-07-31 [PMID: 37527036] (IHC, Mouse)
• Stueven NA, Beauvais DM, Hu R et al. Inhibiting IGF1R-mediated Survival Signaling in Head and Neck Cancer with the Peptidomimetic SSTN(IGF1R) Cancer Research Communications 1/19/2023 [PMID: 36968227]
• Stueven NA, Beauvais DM, Hu R et al. Inhibiting IGF1R-mediated Survival Signaling in Head and Neck Cancer with the Peptidomimetic SSTN(IGF1R) Cancer Research Communications 2023-01-19 [PMID: 36968227]
• Hiroshi YOMOGITA, Hikaru ITO, Kento HASHIMOTO, Akihiko KUDO, Toshiaki FUKUSHIMA, Tsutomu ENDO et al. A possible function of <i>Nik-related kinase</i> in the labyrinth layer of delayed delivery mouse placentas Journal of Reproduction and Development 2/8/2023 [PMID: 36567126]
• CJ Green, M Span, MH Rayhanna, M Perera, ML Day Insulin-like Growth Factor Binding Protein 3 Increases Mouse Preimplantation Embryo Cleavage Rate by Activation of IGF1R and EGFR Independent of IGF1 Signalling Cells, 2022-11-24;11(23):. 2022-11-24 [PMID: 36497022] (Cell Culture, Mouse)
• Y Wang, L Yin, X Sun CircRNA hsa_circ_0002577 accelerates endometrial cancer progression through activating IGF1R/PI3K/Akt pathway J. Exp. Clin. Cancer Res., 2020-08-26;39(1):169. 2020-08-26 [PMID: 32847606] (Western Blot, Human)
• CD Griffiths, LM Bilawchuk, JE McDonough, KC Jamieson, F Elawar, Y Cen, W Duan, C Lin, H Song, JL Casanova, S Ogg, LD Jensen, B Thienpont, A Kumar, TC Hobman, D Proud, TJ Moraes, DJ Marchant IGF1R is an entry receptor for respiratory syncytial virus Nature, 2020-06-03;0(0):. 2020-06-03 [PMID: 32494007] (Neutralization, Human)
• B Haas, V Klinger, C Keksel, V Bonigut, D Kiefer, J Caspers, J Walther, M Wos-Magang, S Weickhardt, G Röhn, M Timmer, R Frötschl, N Eckstein Inhibition of the PI3K but not the MEK/ERK pathway sensitizes human glioma cells to alkylating drugs Cancer Cell Int., 2018-05-04;18(0):69. 5/4/2018 [PMID: 29755294]
• G Lamberti, C Ceccarelli, N Brighi, I Maggio, D Santini, C Mosconi, C Ricci, G Biasco, D Campana Determination of Mammalian Target of Rapamycin Hyperactivation as Prognostic Factor in Well-Differentiated Neuroendocrine Tumors Gastroenterol Res Pract, 2017-10-29;2017(0):7872519. 2017-10-29 [PMID: 29213282] (IHC-P, Human)
• Annika Nordstrand, Sofia Halin Bergström, Elin Thysell, Erik Bovinder-Ylitalo, Ulf H. Lerner, Anders Widmark et al. Inhibition of the insulin-like growth factor-1 receptor potentiates acute effects of castration in a rat model for prostate cancer growth in bone Clinical & Experimental Metastasis 4/26/2017 [PMID: 28447314]
• J Rios-Doria, J Harper, R Rothstein, L Wetzel, J Chesebroug, AM Marrero, C Chen, P Strout, K Mulgrew, KA McGlinchey, R Fleming, B Bezabeh, J Meekin, D Stewart, M Kennedy, P Martin, A Buchanan, N Dimasi, EF Michelotti, RE Hollingswo Antibody-drug conjugates bearing pyrrolobenzodiazepine or tubulysin payloads are immunomodulatory and synergize with multiple immunotherapies Cancer Res, 2017-03-10;0(0):. 2017-03-10 [PMID: 28283653] (Functional Assay, Rat)
• Vincent Le Coz, Chaobin Zhu, Aurore Devocelle, Aimé Vazquez, Claude Boucheix, Sandy Azzi et al. IGF-1 contributes to the expansion of melanoma-initiating cells through an epithelial-mesenchymal transition process Oncotarget 12/13/2016 [PMID: 27764776]
• L R Batista-Si Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages Sci Rep, 2016-06-10;6(0):27632. 2016-06-10 [PMID: 27282338] (Luciferase Reporter Assay, Mouse)
• Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALS Sci Rep, 2016-05-16;6(0):25960. 5/16/2016 [PMID: 27180807]
• Ma J, Guo C, Guo C, Sun Y, Liao T, Beattie U, Lopez F, Chen D, Lashkari K Transplantation of Human Neural Progenitor Cells Expressing IGF-1 Enhances Retinal Ganglion Cell Survival. PLoS ONE, 2015-04-29;10(4):e0125695. 2015-04-29 [PMID: 25923430] (Neutralization, Mouse)
• Tworkoski K, Raab-Traub N LMP1 promotes expression of insulin-like growth factor 1 (IGF1) to selectively activate IGF1 receptor and drive cell proliferation. J Virol, 2014-12-17;89(5):2590-602. 2014-12-17 [PMID: 25520502] (Neutralization, Human)
• Jones R, Watson K, Campbell C, Moorehead R IGF-IR mediated mammary tumorigenesis is enhanced during pubertal development. PLoS ONE, 2014-09-26;9(9):e108781. 2014-09-26 [PMID: 25259518] (IHC, Mouse)
• Mathur , Anjili, Ware , Christop, Davis , Lenora, Gazdar , Adi, Pan , Bo-Sheng, Lutterbach , Bart FGFR2 is amplified in the NCI-H716 colorectal cancer cell line and is required for growth and survival. PLoS ONE, 2014-06-26;9(6):e98515. 2014-06-26 [PMID: 24968263] (Neutralization, Human)
• Charmaine J. Green, Margot L. Day Insulin-like growth factor 1 acts as an autocrine factor to improve early embryogenesis in vitro The International Journal of Developmental Biology 1/1/2013 [PMID: 24623075]
• Annika Nordstrand, Marie Lundholm, Andreas Larsson, Ulf H. Lerner, Anders Widmark, Pernilla Wikström Inhibition of the Insulin-Like Growth Factor-1 Receptor Enhances Effects of Simvastatin on Prostate Cancer Cells in Co-Culture with Bone Cancer Microenvironment 12/1/2013 [PMID: 23335094]
• Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Cancer Res., 2009-03-24;69(7):2996-3003. 2009-03-24 [PMID: 19318572] (Western Blot, Human)
• Yu L, Saile K, Swartz CD, He H, Zheng X, Kissling GE, Di X, Lucas S, Robboy SJ, Dixon D Differential expression of receptor tyrosine kinases (RTKs) and IGF-I pathway activation in human uterine leiomyomas. Mol. Med., 2008-05-01;14(5):264-75. 2008-05-01 [PMID: 18231572] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IGF1R
• Uniprot:
  • P08069()