IGF2BP3 Antibody (8K4H5)

Western Blot: IGF2BP3 Antibody (8K4H5) [NBP3-16205] - Western blot analysis of extracts of various cell lines, using IGF2BP3 antibody (NBP3-16205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry-Paraffin: IGF2BP3 Antibody (8K4H5) [NBP3-16205] - Immunohistochemistry of paraffin-embedded human oophoroma using IGF2BP3 Rabbit mAb (NBP3-16205) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Product Discontinued

IGF2BP3 Antibody (8K4H5) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 2-194 of human IGF2BP3 (O00425). NKLYIGNLSENAAPSDLESIFKDAKIPVSGPFLVKTGYAFVDCPDESWALKAIEALSGKIELHGKPIEVEHSVPKRQRIRKLQIRNIPPHLQWEVLDSLLVQYGVVESCEQVNTDSETAVVNVTYSSKDQARQALDKLNGFQLENFTLKVAYIPDEMAAQQNPLQQPRGRRGLGQRGSSRQGSPGSVSKQKPC
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IGF2BP3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.05% BSA, 50% glycerol, pH7.3
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for IGF2BP3 Antibody (8K4H5)

• CT98
• hKOC
• IGF II mRNA binding protein 3
• IGF2 mRNA-binding protein 3
• IGF-II mRNA-binding protein 3
• IMP-3KH domain-containing protein overexpressed in cancer
• IMP3VICKZ family member 3
• insulin-like growth factor 2 mRNA binding protein 3
• insulin-like growth factor 2 mRNA-binding protein 3
• KH domain containing protein overexpressed in cancer
• KOC1DKFZp686F1078
• VICKZ3cancer/testis antigen 98

Background

The protein encoded by the IGF2BP3 gene is primarily found in the nucleolus, where it can bind to the 5' UTR of theinsulin-like growth factor II leader 3 mRNA and may repress translation of insulin-like growth factor II during latedevelopment. The encoded protein contains several KH domains, which are important in RNA binding and are known to beinvolved in RNA synthesis and metabolism. A pseudogene exists on chromosome 7, and there are putative pseudogenes onother chromosomes. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGF2BP3