IGFBP-2 Antibody (1F6F6)

Western Blot: IGFBP-2 Antibody (1F6F6) [NBP1-28891] - Analysis using IGFBP2 mouse mAb against truncated IGFBP2-His recombinant protein (1) and truncated IGFBP2 (aa40-328)-hIgGFc transfected CHO-K1 cell lysate (2).

Immunohistochemistry-Paraffin: IGFBP-2 Antibody (1F6F6) [NBP1-28891] - Analysis of human lung cancer (A), recturn(B), prostate (C), colon cancer (D) showing cytoplasmic localization using IGFBP2 mouse mAb with DAB staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 1F6F6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IGFBP-2 Antibody (1F6F6) Summary

• Immunogen: Purified recombinant fragment of protein IGFBP-2 (aa180-328) expressed in E. Coli.
• Specificity: Specific for IGFBP2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGFBP2
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for IGFBP-2 Antibody (1F6F6)

• BP2
• IBP2
• IBP-2
• IGF-binding protein 2
• IGFBP2
• IGFBP-2
• IGF-BP53
• insulin-like growth factor binding protein 2 (36kD)
• insulin-like growth factor binding protein 2, 36kDa
• insulin-like growth factor-binding protein 2

Background

IGFBP2 is a member of the ISGBP family which bind various IGFs. IGFBP2 is over expressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The over expression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGFBP-2 Antibody (NBP1-28891)(1)

Publication using NBP1-28891

• Berger M, Hsieh CY, Bakele M et al. Neutrophils express distinct RNA receptors in a non-canonical way. J Biol Chem. 2012-06-01 [PMID: 22532562]
  
  Details:
  TLR8-PE (IMG-321D): Flow (intracellular) + Flow (cell surface), Fig 1A (human neutrophils) & Fig 1B (HL60).2. TLR8 (IMG-321A): IF confocal microscopy, Fig 1C (human neutrophils).3. FITC-conjugated goat anti-rabbit secondary Ab (Cat no 20302), Flow (intracellular) + Flow (cell surface), various Figs.4. FITC-conjugated donkey anti-rat secondary Ab (Cat no 20204), Flow (intracellular) + Flow (cell surface), various Figs.

Bioinformatics

• Gene Symbol: IGFBP2
• Entrez:
  • 3485(Human)