Goat anti-Rat IgG (H+L) Secondary Antibody [Cy5]

Immunofluorescence: Goat anti-Rat IgG Secondary Antibody [Cy5] [CY-4500-NB] - Tonsil (FFPE) was antigen retrieved wtih antigen unmasking solution and stainged with acidic cytokeratin rat primary antibody followed by Cy5 goat anti-rat IgG.

Product Discontinued

Goat anti-Rat IgG (H+L) Secondary Antibody [Cy5] Summary

• Description: Features
• Affinity-purified, ultrapure, high affinity antibody
• Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.
• Unless otherwise specified, antibodies recognizes both heavy and light chains (H+L).
• Optimally labeled with Cy5 to provide the brightest label for fluorescence microscopy.
• Supplied in solution
• Excitation: 650 nm
• Emission: 670 nm
• Color: Far Red
• Immunogen: Rat IgG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • In-situ Hybridization
• Application Notes: Recommended concentration range for use 5-20 ug/ml..

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10 mM HEPES, 0.15 M NaCl, pH 7.5
• Preservative: 0.08% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Notes

This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.

Background

The anti-rat Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis. Unless otherwise specified, our antibodies will recognize both heavy and light chains (H+L).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.