Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC]

Product Discontinued

Goat anti-Rabbit IgG (H+L) Secondary Antibody [FITC] Summary

• Immunogen: Rabbit IgG. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to fluorescein isothiocyanate (FITC).
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No was detected against nonimmunoglobulin serum proteins. This may cross react with IgG from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: This antibody can be used for IHC-Paraffin and ICC.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS containing 0.2 BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (NB730-F)(6)

Publications using NB730-F

• Kim Jh, Oh Ct, Kwon Tr et Al. Inhibition of melanogenesis by sodium 2-mercaptoethanesulfonate Korean J. Physiol. Pharmacol. 2020-03-01 [PMID: 32140038] (ICC/IF)
• Yang JS, Wang CM, Su CH, Ho HC. Eudesmin attenuatesHelicobacter pylori-induced epithelial autophagy and apoptosis and leads to eradication ofH. pyloriinfection. Exp Ther Med. 2018-03-01 [PMID: 29456644] (ICC/IF)
• Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014-01-01 [PMID: 25587023] (ICC/IF)
• Guest CB, Hartman ME, O'Connor JC et al. Phagocytosis of cholesteryl ester is amplified in diabetic mouse macrophages and is largely mediated by CD36 and SR-A. PLoS One. 2007-06-06 [PMID: 17551591]
• Haehl E Endothelial-cell-induced radioresistance in glioblastoma Thesis 2021-01-01 (ICC/IF)
• Grimaldi P, Orlo P, Di Siena S et al. The endocannabinoid system pivotal role of the CB2 receptor in mouse spermatogenesis. PNAS;106(27):11131-11136. 2009-01-01 [PMID: 19541620] (ICC/IF)

Reviews for IgG Antibody (NB730-F) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Arun Satelli IF Human 10/14/2014

Summary

• Application: Immunofluorescence
• Sample Tested: Human colon cancer cell line HT29
• Species: Human

reviewed by: Arun Satelli IHC-P Human 10/14/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human colon tissue
• Species: Human

reviewed by: Arun Satelli Flow Human 10/14/2014

Summary

• Application: Flow Cytometry
• Sample Tested: Human colon cancer cell line HT29
• Species: Human