Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73726] - Western Blot of Goat anti-Monkey IgM Heavy Chain Secondary antibody [HRP]. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane.Primary antibody: none.Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT.Block for 30 min at RT.Observed size: 72 kDa for Rabbit IgG. Other band(s): none.

N/A: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73726]

Western Blot of Peroxidase conjugated Goat anti-Monkey IgM mu antibody. Lane 1: Monkey IgM. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min at RT. Observed size: 72 kDa for Rabbit IgG. Other band(s): none.

• Reactivity: Pm
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: LYOPH

Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Monkey IgM and Monkey Serum
• Immunogen: Monkey IgM whole molecule
• Specificity: No reaction was observed against other Monkey heavy or light chain proteins.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHM
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:65000 - 1:160000
  • Immunohistochemistry 1:500 -1:2500
  • Western Blot 1:1000 - 1:5000
• Application Notes: This product has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water (or equivalent).

Alternate Names for Goat anti-Monkey IgM Heavy Chain Secondary Antibody [HRP]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgM Heavy Chain Antibody (NBP1-73726)(1)

Publication using NBP1-73726

• de Alwis R, Zellweger RM, Chua E et al. Systemic inflammation, innate immunity and pathogenesis after Zika virus infection in cynomolgus macaques are modulated by strain-specificity within the Asian lineage Emerg Microbes Infect 2021-06-14 [PMID: 34120576] (ELISA)
  
  Details:
  This citation used the HRP format of this antibody.

FAQs for IgM Heavy Chain Antibody (NBP1-73726). (Showing 1 - 2 of 2 FAQ).

1. Can this antibody be used with samples from the Rhesus monkey or cross re-activity with Rhesus monkey ?
   • This product is generally specific for Monkey IgM's which includes mostly Old World monkeys, like: Macaque, Cynomologus, Rhesus, Baboon, Chimpanzee, Great Ape and any other African species. Reactivity to New World primates has not yet been determined.
2. Will this antibody cross react with Human IgM?
   • Although it has not been specifically tested for human IgM, this antibody is predicted to cross react with human IgM. 

Bioinformatics

• Gene Symbol: IGHM