Goat anti-Chicken IgY (H+L) Secondary Antibody [HRP] (Pre-adsorbed)

• Reactivity: Ch
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Format: Pre-adsorbed
• Concentration: 0.5 mg/ml

Goat anti-Chicken IgY (H+L) Secondary Antibody [HRP] (Pre-adsorbed) Summary

• Immunogen: Chicken IgG (IgY)-heavy and light chain
• Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit, and rat immunosorbents to remove cross reactive Antibodies.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin 1:200-1:5000
  • Western Blot 1:1000- 1:30000
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS) containing 0.2%BSA
• Preservative: 0.05% Pro-Clean 400
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgY (H+L) Antibody (NB7303)(2)

Publications using NB7303

• Iqbal Dulloo, Michael Tellier, Clémence Levet, Anissa Chikh, Boyan Zhang, Diana C Blaydon, Catherine M Webb, David P Kelsell, Matthew Freeman Cleavage of the pseudoprotease iRhom2 by the signal peptidase complex reveals an ER-to-nucleus signaling pathway. Molecular cell 2024-01-18 [PMID: 38183983]
• B Sieber, F Lu, SM Stribbling, AG Grieve, AJ Ryan, M Freeman iRhom2 regulates ERBB signalling to promote KRAS-driven tumour growth of lung cancer cells Journal of Cell Science, 2022-09-08;0(0):. 2022-09-08 [PMID: 35971826]