Human IL-10 ELISA Kit (Colorimetric)

ELISA: IL-10 ELISA Kit [NBP1-89871] - Representative standard curve for human IL-10 ELISA. Human IL-10 was diluted in serial 2-fold steps in Assay Buffer (1x)

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution
• Standard Curve Range: 3.15 - 200 pg/mL
• Sensitivity: 1.0 pg/mL

Human IL-10 ELISA Kit (Colorimetric) Summary

• Description: The human IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-10.
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-10 positive serum. There was no crossreactivity detected.
• Standard Curve Range: 3.15 - 200 pg/mL
• Sensitivity: 1.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 5.6%
• Intra-Assay: CV% < 3.2
• Spike Recovery: The recovery ranged from 81% to 106% with an overall mean recovery of 97%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL10

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IL-10 ELISA Kit (Colorimetric)

• CSIF
• CSIFMGC126450
• Cytokine synthesis inhibitory factor
• GVHDS
• IL10
• IL-10
• IL10A
• IL-10MGC126451
• interleukin 10
• interleukin-10
• TGIF

Background

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IL-10 ELISA Kit (NBP1-89871). (Showing 1 - 1 of 1 FAQ).

1. Could you let me know whether you have used baculovirus to produce this product? 
   • For this kit, all proteins are expressed in an E.coli expression system. None of the components are expressed in baculovirus.

Bioinformatics

• Gene Symbol: IL10