IL-10R alpha Antibody (3F9-2)

Product Discontinued

IL-10R alpha Antibody (3F9-2) Summary

• Immunogen: Soluble human CD210
• Specificity: Recognizes human CD210, a 90-110kD glycoprotein, also known as Interleukin-10 receptor (IL-10R). CD210 is expressed on T cells, NK cells, monocytes and dendritic cells, although surface expression may be low. Recent work has reported an increase in intracellular expression of CD210 during dendritic cell maturation, but a reduction in surface CD210 expression (3). This clone is reported to function as a blocking antibody. Immunogen: Soluble human CD210
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: IL10RA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:50
• Application Notes: Flow cytometry: Use 10ul of the suggested working dilution (1/10-1/50) to label 1x 106 cells in 100ul. Due to low expression of CD210, sensitive staining techniques may be required.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for IL-10R alpha Antibody (3F9-2)

• CD210 antigen
• CDw210a
• HIL-10R
• IBD28
• IL-10 R alpha
• IL-10 receptor subunit alpha
• IL10R alpha
• IL-10R subunit 1
• IL-10R1
• IL10RA
• IL-10Ra
• IL10RIL-10R subunit alpha
• interleukin 10 receptor, alpha
• interleukin-10 receptor alpha chain
• Interleukin-10 receptor subunit 1
• interleukin-10 receptor subunit alpha

Background

CD210 is a 90-110 kD IL-10 receptor. It is a class II cytokine receptor expressed on thymocytes, T cells, B cells, NK cells, monocytes and macrophages. Ligand binding of CD210 induces Jak1 and Tyk, resulting in STAT1 and STAT3 activation. IL-10 receptor stimulation results in the inhibition of cytokine production and the costimulation of B cell proliferation and differentiation. The only known ligand for this receptor is IL-10.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-10R alpha Antibody (NBP1-42534)(1)

Publication using NBP1-42534

• Beck D, Zobel J, Barber R et al. Synthetic lethal screen demonstrates that a JAK2 inhibitor suppresses a BCL6 dependent IL10RA/JAK2/STAT3 pathway in high grade B-cell lymphoma. J. Biol. Chem. 2016-06-06 [PMID: 27268052] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: IL10RA
• Entrez:
  • 3587(Human)
• Uniprot:
  • Q13651()