IL-12 R beta 1 Antibody (69310) [Phycoerythrin]

Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 5 µg/mL PHA for 48 hours or (B) untreated were stained with Mouse Anti-Human IL-12 R beta 1 PE-conjugated Monoclonal Antibody (Catalog # FAB839P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Daudi cells were stained with Mouse Anti-Human IL‑12 R beta 1 PE‑conjugated Monoclonal Antibody (Catalog # FAB839P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

IL-12 R beta 1 Antibody (69310) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 1
  Cys24-Glu540
  Accession # P42701
• Specificity: Detects human IL-12 R beta 1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-12 R beta 1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL12RB1
• Purity Statement: Protein A or G purified from ascites

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12 R beta 1 Antibody (69310) [Phycoerythrin]

• CD212 antigen
• CD212
• IL-12 R beta 1
• IL-12 receptor beta component
• IL-12 receptor subunit beta-1
• IL12R beta 1
• IL-12R subunit beta-1
• IL12R
• IL12RB
• IL12RB1
• IL-12Rb1
• IL-12R-BETA1
• IL-12R-beta-1
• interleukin 12 receptor, beta 1
• interleukin-12 receptor beta-1 chain
• interleukin-12 receptor subunit beta-1
• MGC34454

Background

Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta 1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta 2 is more restricted and appears to be limited to Th2 cells.

1. Gately, M.K. et al. (1998) Annu. Rev. Immunol. 16:495.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-12 R beta 1 Antibody (FAB839P)(5)

Publications using FAB839P

• M Noack, P Miossec Synoviocytes and skin fibroblasts show opposite effects on IL-23 production and IL-23 receptor expression during cell interactions with immune cells Arthritis Research & Therapy, 2022-09-10;24(1):220. 2022-09-10 [PMID: 36088336] (Flow Cytometry, Human)
• TM Hummel, T Ackfeld, M Schönberg, G Ciupka, F Schulz, A Oberdoerst, J Grötzinger, J Scheller, DM Floss Synthetic deletion of the Interleukin 23 receptor (IL-23R) stalk region led to autonomous IL-23R homodimerization and activation Mol. Cell. Biol., 2017-08-11;0(0):. 8/11/2017 [PMID: 28630278]
• Hodge , Lucy S, Ziesmer , Steve C, Yang , Zhi Zhan, Secreto , Frank J, Gertz , Morie A, Novak , Anne J, Ansell , Stephen IL-21 in the bone marrow microenvironment contributes to IgM secretion and proliferation of malignant cells in Waldenstrom macroglobulinemia. Blood, 2012-09-13;120(18):3774-82. 2012-09-13 [PMID: 22976953] (Flow Cytometry, Human)
• Cocco C, Di Carlo E, Zupo S Complementary IL-23 and IL-27 anti-tumor activities cause strong inhibition of human follicular and diffuse large B-cell lymphoma growth in vivo. Leukemia, 2011-12-23;26(6):1365-74. 2011-12-23 [PMID: 22193967] (Flow Cytometry, Human)
• Fieschi C, Bosticardo M, de Beaucoudrey L, Boisson-Dupuis S, Feinberg J, Santos OF, Bustamante J, Levy J, Candotti F, Casanova JL A novel form of complete IL-12/IL-23 receptor beta1 deficiency with cell surface-expressed nonfunctional receptors. Blood, 2004-06-03;104(7):2095-101. 2004-06-03 [PMID: 15178580] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: IL12RB1
• Uniprot:
  • P42701()