IL-13R alpha 2 Antibody [Unconjugated]

IL-13 Ra2 was detected in immersion fixed mouse splenocytes using Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL-13 Ra2 was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse IL-13 Ra2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF539) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-13R alpha 2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑13 R alpha 2
  Leu22-Lys334
  Accession # O88786
• Specificity: Detects mouse IL-13 R alpha 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-13 R alpha 2 is observed and less than 1% cross-reactivity with rhIL-5R alpha , rhIL-5R beta , rhIL-9 R, and rhIL-4 R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL13RA2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-13R alpha 2 Antibody [Unconjugated]

• cancer/testis antigen 19
• CD213a2 antigen
• CD213a2
• CT19
• IL-13 R alpha 2
• IL-13 receptor subunit alpha-2
• IL13BP
• IL13R alpha 2
• IL-13R subunit alpha-2
• IL13R
• IL-13R
• IL13RA2
• IL-13Ra2
• IL-13R-alpha-2
• interleukin 13 binding protein
• interleukin 13 receptor alpha 2 chain
• interleukin 13 receptor, alpha 2
• interleukin-13 receptor subunit alpha-2
• Interleukin-13-binding protein

Background

Two type1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha , IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

The mouse IL-13 R alpha 2 cDNA encodes a 383 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 313 residue extracellular domain, a 22 aa residue transmembrane region, and a 27 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 2 share 59% aa sequence identity. The extracellular domain of IL-13 R alpha 2 is also closely related to that of IL-13 R alpha 1. However, the cytoplasmic domain of IL-13 R alpha 2 lacks the box 1 and box 2 signaling motif and is much shorter than that of IL-13 R alpha 1, suggesting that the two receptors are functionally distinct. IL-13 R alpha 1 has been shown to combine with IL-4 R alpha to form a high-affinity receptor complex capable of transducing both an IL-4-dependent and an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated. The amino-terminal 27 amino acid residues of mouse IL-13 R alpha 2 are identical to that of a soluble mouse IL-13 binding protein purified from mouse serum and urine. Recombinant mouse IL-13 R alpha /Fc chimera has been shown to bind IL-13 with high affinity and is a potent IL-13 antagonist.

1. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-13R alpha 2 Antibody (AF539)(12)

Publications using AF539

• S Xiao, Z Lu, M Steinhoff, Y Li, T Buhl, M Fischer, W Chen, W Cheng, R Zhu, X Yan, H Yang, Y Liu, Y Dou, W Wang, J Wang, J Meng Innate immune regulates cutaneous sensory IL-13 receptor alpha 2 to promote atopic dermatitis Brain, Behavior, and Immunity, 2021-08-13;98(0):28-39. 2021-08-13 [PMID: 34391816] (Western Blot, Mouse)
• RK Radhakrish, RS Thandi, D Tripathi, P Paidipally, MK McAllister, S Mulik, B Samten, R Vankayalap BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice JCI Insight, 2020-03-12;5(5):. 2020-03-12 [PMID: 32161191] (IHC, Mouse)
• Shimpei Matsui, Koji Okabayashi, Masashi Tsuruta, Kohei Shigeta, Ryo Seishima, Takashi Ishida et al. Interleukin‐13 and its signaling pathway is associated with obesity‐related colorectal tumorigenesis Cancer Science 2019-07-01 [PMID: 31099450]
• MA Hamid, RJ Jackson, S Roy, M Khanna, C Ranasinghe Unexpected involvement of IL-13 signalling via a STAT6 independent mechanism during murine IgG2a development following viral vaccination Eur. J. Immunol., 2018-05-10;0(0):. 2018-05-10 [PMID: 29569714] (Flow Cytometry, Mouse)
• T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice World J. Gastroenterol., 2017-04-07;23(13):2294-2307. 2017-04-07 [PMID: 28428709] (IHC, Neutralization, Mouse)
• IL-13R?2 uses TMEM219 in chitinase 3-like-1-induced signalling and effector responses Nat Commun, 2016-09-15;7(0):12752. 9/15/2016 [PMID: 27629921]
• Zheng S, Lu Q, Xu Y, Wang X, Shen J, Wang W GdCl3 Attenuates Schistosomiasis japonicum Egg-Induced Granulomatosis Accompanied by Decreased Macrophage Infiltration in Murine Liver. PLoS ONE, 2015-08-28;10(8):e0132222. 2015-08-28 [PMID: 26317423] (IHC-P, Mouse)
• Ferreira T, de Arantes A, do Nascimento C, Olsen P, Trentin P, Rocco P, Hogaboam C, Puri R, Martins M, Silva P IL-13 immunotoxin accelerates resolution of lung pathological changes triggered by silica particles in mice. J Immunol, 2013-10-16;191(10):5220-9. 10/16/2013 [PMID: 24133168]
• Chen W, Tabata Y, Gibson AM, Daines MO, Warrier MR, Wills-Karp M, Hershey GK Matrix metalloproteinase 8 contributes to solubilization of IL-13 receptor alpha2 in vivo. J. Allergy Clin. Immunol., 2008-08-09;122(3):625-32. 2008-08-09 [PMID: 18694590] (ELISA Development, Flow Cytometry, Western Blot, Mouse)
• Pierce J, Rir-Sima-Ah J, Estrada I, Wilder J, Strasser A, Tesfaigzi Y Loss of pro-apoptotic Bim promotes accumulation of pulmonary T lymphocytes and enhances allergen-induced goblet cell metaplasia. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-06-16;291(5):L862-70. 2006-06-16 [PMID: 16782750] (IHC-P, Mouse)
• Daines MO, Tabata Y, Walker BA, Chen W, Warrier MR, Basu S, Hershey GK Level of expression of IL-13R alpha 2 impacts receptor distribution and IL-13 signaling. J. Immunol., 2006-06-15;176(12):7495-501. 2006-06-15 [PMID: 16751396] (Flow Cytometry, Mouse)
• Jakubzick C, Choi ES, Kunkel SL, Joshi BH, Puri RK, Hogaboam CM Impact of interleukin-13 responsiveness on the synthetic and proliferative properties of Th1- and Th2-type pulmonary granuloma fibroblasts. Am. J. Pathol., 2003-05-01;162(5):1475-86. 2003-05-01 [PMID: 12707030] (ICC, Mouse)

Bioinformatics

• Gene Symbol: IL13RA2
• Uniprot:
  • O88786()