IL-15 Antibody (34593) [Unconjugated]

Recombinant Human IL‑15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑15 Monoclonal Antibody (Catalog # MAB247). The ND50 is typically0.007-0.042 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 34593
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-15 Antibody (34593) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL-15
  Asn49-Ser162
  Accession # P40933
• Specificity: Detects human IL-15 in Western blots. In Western blots, no cross-reactivity with recombinant human IL‑2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL15
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.007-0.042 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-15 Antibody (34593) [Unconjugated]

• IL15
• IL-15
• IL-15MGC9721
• interleukin 15
• interleukin-15

Background

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8⁺ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

1. Grabstein, K. et al. (1994) Science 264:965.
2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
5. Giri, J.G. et al. (1995) EMBO 14:3654.
6. Giri, J. et al. (1994) EMBO J. 13:2822.
7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
8. Dubois, S. et al. (2002) Immunity 17:537.
9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-15 Antibody (MAB247)(22)

Publications using MAB247

• Vijayashree Mysore, Xavier Cullere, Matthew L. Settles, Xinge Ji, Michael W. Kattan, Michaël Desjardins et al. Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens Med (N Y) 9/1/2021 [PMID: 34414383]
• Moriah J. Castleman, Stephanie M. Dillon, Tezha A. Thompson, Mario L. Santiago, Martin D. McCarter, Edward Barker et al. Gut Bacteria Induce Granzyme B Expression in Human Colonic ILC3s In Vitro in an IL-15–Dependent Manner The Journal of Immunology 6/15/2021 [PMID: 34117105]
• Catherine B. Xie, Bo Jiang, Lingfeng Qin, George Tellides, Nancy C. Kirkiles-Smith, Dan Jane-wit et al. Complement-activated interferon-gamma –primed human endothelium transpresents interleukin-15 to CD8+ T cells Journal of Clinical Investigation 5/26/2020 [PMID: 32191642]
• L Swadling, LJ Pallett, MO Diniz, JM Baker, OE Amin, KA Stegmann, AR Burton, NM Schmidt, A Jeffery-Sm, N Zakeri, K Suveizdyte, F Froghi, G Fusai, WM Rosenberg, BR Davidson, A Schurich, AK Simon, MK Maini Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence Cell Rep, 2020-01-21;30(3):687-698.e6. 2020-01-21 [PMID: 31968246] (Flow Cytometry, Human)
• Ahmet Eken, Zehra Okus, Serife Erdem, Zehra Busra Azizoglu, Yesim Haliloglu, Ayten Bicer et al. IL-15 negatively regulates curdlan-induced IL-23 production by human monocyte-derived dendritic cells and subsequent Th17 response Northern Clinics of Istanbul 10/24/2019 [PMID: 31909384]
• Agnès Quéméner, Sébastien Morisseau, Rui P. Sousa, Kilian Trillet, Mike Maillasson, Isabelle Leray et al. IL-15R alpha membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signaling Journal of Cell Science 3/1/2020 [PMID: 31653781]
• DC Watson, BC Yung, C Bergamasch, B Chowdhury, J Bear, D Stellas, A Morales-Ka, JC Jones, BK Felber, X Chen, GN Pavlakis Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes J Extracell Vesicles, 2018-02-28;7(1):1442088. 2018-02-28 [PMID: 29535850] (Transmission Electron Microsco, Human)
• Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara et al. Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium eLife 2017-12-11 [PMID: 29227245] (Bioassay, Human)
• Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara et al. Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium eLife 12/11/2017 [PMID: 29227245]
• Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015-07-16;290(37):22338-51. 2015-07-16 [PMID: 26183780] (Neutralization, Human)
• Huaqin Yuan, Xiaoxin Meng, Wenjie Guo, Peifen Cai, Wanshuai Li, Qian Li et al. Transmembrane-Bound IL-15–Promoted Epithelial-Mesenchymal Transition in Renal Cancer Cells Requires the Src-Dependent Akt/GSK-3 beta / beta -Catenin Pathway12 Neoplasia 5/27/2015 [PMID: 26025664]
• Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival. PLoS ONE, 2012-07-09;7(7):e40620. 2012-07-09 [PMID: 22792388] (Neutralization, Human)
• Vinay V. Vyas, Dominic Esposito, Terry L. Sumpter, Trevor L. Broadt, James Hartley, George C. Knapp IV et al. Clinical manufacturing of recombinant human interleukin 15. I. Production cell line development and protein expression in E. coli with stop codon optimization Biotechnology Progress 3/1/2012 [PMID: 22162520]
• Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha. Blood, 2011-02-08;117(15):4032-40. 2011-02-08 [PMID: 21304101] (Neutralization, Human)
• D K Finch, A Midha, C L Buchanan, D Cochrane, R I Craggs, S Cruwys et al. Identification of a potent anti-IL-15 antibody with opposing mechanisms of action in vitro and in vivo British Journal of Pharmacology 1/1/2011 [PMID: 20942844]
• Perrot I, Deauvieau F, Massacrier C TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA. J. Immunol., 2010-07-16;185(4):2080-8. 2010-07-16 [PMID: 20639488] (Neutralization, Mouse)
• Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils. Arthritis Rheum., 2006-07-01;54(7):2096-108. 2006-07-01 [PMID: 16802344] (Neutralization, Human)
• Dean GA, Barger A, Lavoy A Cloning and expression of feline interleukin 15. Cytokine, 2005-01-21;29(2):77-83. 2005-01-21 [PMID: 15598442] (ICC, Western Blot, Feline)
• Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs. Proc. Natl. Acad. Sci. U.S.A., 2004-11-09;101(47):16606-11. 2004-11-09 [PMID: 15536127] (Flow Cytometry, Human)
• Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate. J. Immunol., 2004-07-15;173(2):1463-76. 2004-07-15 [PMID: 15240743] (Neutralization, Human)
• Briard D, Brouty-Boye D, Azzarone B, Jasmin C Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15. J. Immunol., 2002-05-01;168(9):4326-32. 2002-05-01 [PMID: 11970974] (Neutralization, Human)
• Corinti S, la Sala A, Pastore S Regulatory activity of autocrine IL-10 on dendritic cell functions. J. Immunol., 2001-04-01;166(7):4312-8. 2001-04-01 [PMID: 11254683] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL15
• Uniprot:
  • P40933()