Recombinant Human IL-15 Animal-Free Protein

SDS-Page: Recombinant Human IL-15 Animal-Free Protein [NBP2-76207] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.

Bioactivity: Recombinant Human IL-15 Animal-Free Protein [NBP2-76207] - IL-15 induced proliferation of Mo7e cells.

Product Discontinued

Recombinant Human IL-15 Animal-Free Protein Summary

• Description: An un-tagged bioactive recombinant protein corresponding to Human IL-15
  
  

  Source: E. coli

  Amino Acid Sequence: MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS

  Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

  Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage
  
  

  Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
• Specificity: IL-15 is a monomer, 12.9 kDa (115 aa)
• Preparation Method: Protein is derived from genetically modified E. coli.
• Details of Functionality: CTLL-2 cell proliferation, >2 x 10^5 units/mg (ED50 acceptance criteria are <5 ng/mL).
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL15
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 12.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium bicarbonate, pH 8.5
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human IL-15 Animal-Free Protein

• IL15
• IL-15
• IL-15MGC9721
• interleukin 15
• interleukin-15

Background

IL-15 (14-15 kD) is a member of the four alpha-helical bundle family of cytokines. It is very similar to IL-2, except that IL-15 has an IL-15 alpha receptor subunit. IL-15 plays an important role in the growth and differentiation of T and B lymphocytes, natural killer cells, macrophages, and monocytes as well as activation of a number of important intracellular signaling molecules. This implies that IL-15 could be essential for the immune responses, allograft rejection, and the pathogenesis of autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: IL15