Rat IL-17E/IL-25 ELISA Kit (Colorimetric)

ELISA: Rat IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06783] - Samples were spiked with high concentrations of Rat IL-17E/IL-25 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat IL-17E/IL-25 ELISA Kit (Colorimetric) [NBP3-06783] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL

Rat IL-17E/IL-25 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Rat IL-17E/IL-25 in samples. No significant cross-reactivity or interference between Rat IL-17E/IL-25 and analogues was observed.
• Standard Curve Range: 15.63 - 1000 pg/mL
• Sensitivity: 9.38 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.72
• Intra-Assay: 4.8
• Spike Recovery: 84 - 106 %
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IL25

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat IL-17E/IL-25 ELISA Kit (Colorimetric)

• IL17E
• IL-17E
• IL25
• IL-25
• interleukin 25
• Interleukin-17E
• interleukin-25

Background

Recently, a number of cytokines belonging to the interleukin (IL)-17 family have been identified. These are termed as IL-17B, IL-17C (1) and IL-17E (2). IL-17 is a potent proinflammatory cytokine that plays roles in a number of diseases including rheumatoid arthritis (3), multiple sclerosis (4), and promotion of tumor growth (5). IL-17B, C, and E like IL-17 are able to induce proinflammatory responses. However, they do not bind to the IL-17 receptor suggesting that additional IL-17R related receptor might exist. Receptor for IL-17B and IL-17E has been independently isolated by Shi, et al (6) and Lee, et al. (2) and has been designated as EV127 (in mouse) and IL-17Rh1 (in human), respectively. IL-17E induces activation of NF-kB pathway and like IL-17 also induces production of IL-8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: IL25