IL-17RC Antibody

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL‑17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Recombinant Human IL‑17F (Catalog # 1335-INS) stimulates IL‑6 secretion in the NIH‑3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL‑6 Quantikine ELISA Kit (Catalog # M6000B). IL‑6 secre­tion elicited by Recombinant Human IL‑17F (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270). The ND50 is typically 0.6-3.6 µg/mL.

• Reactivity: Mu
• Applications: WB, Flow, B/N, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-17RC Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RC
  Leu21-Trp465
  Accession # AAH04759
• Specificity: Detects mouse IL-17 RC in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-17 RC is observed and less than 5% cross-reactivity with recombinant mouse (rm) IL‑17 RD and rmIL-17B R is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17RC
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.6-3.6 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17RC Antibody

• FLJ95963
• FLJ96005
• IL-17 RC
• IL-17 receptor C
• IL-17 RL
• IL17F receptor
• IL17RC
• IL-17RC
• IL17Rhom
• IL17RL
• IL-17RL
• IL17-RL
• interleukin 17 receptor C
• interleukin-17 receptor C
• Interleukin-17 receptor homolog
• Interleukin-17 receptor-like protein
• MGC10763
• zcytoR14

Background

IL-17 receptor C (IL-17 RC; also known as IL-17 RL) is an 85‑110 kDa member of the IL-17 receptor family. This is one of five families, termed IL-17 RA, B, C, D and E, that comprise the cytokine receptor superfamily (1‑6). Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17A, while IL-17 RB is reported to bind IL-17B and IL-17E (2, 4). Mouse IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. Full-length IL-17 RC is synthesized as a 674 amino acid (aa) precursor. It contains a 21 aa signal sequence, a 419 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 213 aa cytoplasmic region. There are multiple potential N‑linked glycosylation sites in the ECD and potential phosphorylation sites in the cytoplasmic tail. Four mouse variants have been identified that have been designated mIL-17 RC (7). The isoform expressed here as an R&D product is an unusual 567 aa form (8). Its precursor contains a 20 aa signal sequence, a 444 aa extracellular region, a 20 aa transmembrane segment and an 83 aa cytoplasmic tail. When compared to the full length mouse IL-17 RC form, this expressed isoform’s extracellular region shows absolute aa identity, save for an additional 24 aa insert. In the cytoplasmic region, it is highly divergent and shows virtually no aa identity (8‑9). The extracellular region of mouse IL-17 RC shows about 70% aa identity to the equivalent region in human IL-17 RC isoform # 3. IL-17 RC is the cognate receptor for IL-17F (7). In humans, IL-17 RC binds IL-17A with similar affinity, and with IL-17 RA, it forms a definitive receptor for both IL-17A and IL-17F (7). The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL-17 RA homodimer (4, 7, 10, 11). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
4. Shen, F. and S.L. Gaffen (2008) Cytokine 41:92.
5. You, Z. et al. (2006) Cancer Res. 66:175.
6. You, Z. et al. (2007) Neoplasia 9:464.
7. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
8. GenBank Accession # AAH04759.
9. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
10. Toy, D. et al. (2006) J. Immunol. 177:36.
11. Haudenschild, D.R. et al. (2006) Prostate 66:1268.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17RC Antibody (AF2270)(6)

Publications using AF2270

• D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology Nature Immunology, 2022-10-21;0(0):. 2022-10-21 [PMID: 36271145] (Flow Cytometry, Mouse)
• T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death Cell. Signal., 2020-06-15;73(0):109690. 2020-06-15 [PMID: 32553549] (Neutralization, Western Blot, Mouse)
• Yoshinari Nakatsuka, Alexis Vandenbon, Takashi Mino, Masanori Yoshinaga, Takuya Uehata, Xiaotong Cui et al. Pulmonary Regnase-1 orchestrates the interplay of epithelium and adaptive immune systems to protect against pneumonia Mucosal Immunology 7/1/2018 [PMID: 29695841]
• S Das, M Raundhal, J Chen, TB Oriss, R Huff, JV Williams, A Ray, P Ray Respiratory syncytial virus infection of newborn CX3CR1-deficent mice induces a pathogenic pulmonary innate immune response JCI Insight, 2017-09-07;2(17):. 2017-09-07 [PMID: 28878128] (Neutralization, Mouse)
• Chen K, McAleer JP, Lin Y, Paterson DL, Zheng M, Alcorn JF, Weaver CT, Kolls JK Th17 cells mediate clade-specific, serotype-independent mucosal immunity. Immunity, 2011-12-23;35(6):997-1009. 2011-12-23 [PMID: 22195749] (Neutralization, Mouse)
• Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses. J. Immunol., 2009-06-01;182(11):7119-30. 2009-06-01 [PMID: 19454709] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL17RC
• Uniprot:
  • AAH04759()