IL-2 Antibody (5334) [Unconjugated]

Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202). The ND50 is typically 0.015-0.03 µg/mL.

Recombinant Human IL‑2 (Catalog # 202-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑2 Monoclonal Antibody (Catalog # MAB202). The ND50 is typically 0.015-0.03 µg/mL.

IL-2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA using Mouse Anti-Human IL-2 Monoclonal Antibody (Catalog # MAB202) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: B/N, CyTOF-ready, Flow, ICC/IF
• Clone: 5334
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-2 Antibody (5334) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IL‑2
  Ala21-Thr153
  Accession # NP_000577
• Specificity: Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.015-0.03 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody (5334) [Unconjugated]

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Interleukin 2 (IL-2) is a cytokine that stimulates the growth and differentiation of B cells, T cells, NK cells, and monocyte/macrophages. It functions through the heterotrimeric IL-2 receptor comprising alpha , beta , and gamma chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-2 Antibody (MAB202)(26)

Publications using MAB202

• Moore, GL;Zeng, VG;Diaz, JE;Bonzon, C;Avery, KN;Rashid, R;Qi, J;Nam, DH;Jacinto, J;Dragovich, MA;Kim, YK;Balcazar, KP;Bakhit, CG;Eivazi, A;Nguyen, H;Muchhal, US;Szymkowski, DE;Desjarlais, JR;Hedvat, M; A B7-H3-targeted CD28 bispecific antibody enhances the activity of anti-PD1 and CD3 T-cell engager immunotherapies Molecular cancer therapeutics 2024-09-20 [PMID: 39301613] (Neutralization, Human)
• Zheng N, Fang J, Xue G et al. Induction of tumor cell autosis by myxoma virus-infected CAR-T and TCR-T cells to overcome primary and acquired resistance Cancer cell 8/15/2022 [PMID: 36027915]
• M Ukita, J Hamanishi, H Yoshitomi, K Yamanoi, S Takamatsu, A Ueda, H Suzuki, Y Hosoe, Y Furutake, M Taki, K Abiko, K Yamaguchi, H Nakai, T Baba, N Matsumura, A Yoshizawa, H Ueno, M Mandai CXCL13-producing CD4+ T cells accumulate in early phase of tertiary lymphoid structures in ovarian cancer JCI Insight, 2022-06-22;0(0):. 2022-06-22 [PMID: 35552285] (Neutralization, Human)
• Girija Goyal, Pranav Prabhala, Gautam Mahajan, Bruce Bausk, Tal Gilboa, Liangxia Xie et al. Ectopic Lymphoid Follicle Formation and Human Seasonal Influenza Vaccination Responses Recapitulated in an Organ‐on‐a‐Chip Advanced Science 2022-05-01 [PMID: 35289122]
• Rosemina Merchant, Carole Galligan, Manjunatha Ankathatti Munegowda, L Bruce Pearce, Peter Lloyd, Paul Smith et al. Fine-tuned long-acting interleukin-2 superkine potentiates durable immune responses in mice and non-human primate Journal for ImmunoTherapy of Cancer 2022-01-19 [PMID: 35058325]
• Y Rodríguez-, LG Batista-Ro, A Llopiz-Arz, P Puente-Pér, R Martínez-C, J Castro-Vel, A Santos-Sav Immunogenicity profile in African green monkeys of a vaccine candidate based on a mutated form of human Interleukin-15 BMC immunology, 2021-12-18;22(1):79. 2021-12-18 [PMID: 34922462] (Neutralization, Mouse)
• Hiroshi Kato, Andras Perl Double-Edged Sword: Interleukin-2 Promotes T Regulatory Cell Differentiation but Also Expands Interleukin-13- and Interferon-gamma -Producing CD8+ T Cells via STAT6-GATA-3 Axis in Systemic Lupus Erythematosus Frontiers in Immunology 2021-03-02 [PMID: 33763079]
• D Sahin, N Arenas-Ram, M Rath, U Karakus, M Hümbelin, M van Gogh, L Borsig, O Boyman An IL-2-grafted antibody immunotherapy with potent efficacy against metastatic cancer Nature Communications, 2020-12-22;11(1):6440. 2020-12-22 [PMID: 33353953] (ELISA Detection, Human, Mouse)
• Valentin Haug, Branislav Kollar, Sotirios Tasigiorgos, Yori Endo, Martin Kauke, Ali-Farid Safi et al. Hypothermic Ex Situ Perfusion of Human Limbs With Acellular Solution for 24 Hours Transplantation 2020-09-01 [PMID: 32168040]
• A Güler, ML Venegas, E Adankwah, E Mayatepek, N Nausch, M Jacobsen Suppressor of cytokine signaling 3 is crucial for interleukin-7 receptor re-expression after T-cell activation and interleukin-7 dependent proliferation Eur. J. Immunol., 2019-11-14;0(0):. 2019-11-14 [PMID: 31621896] (Neutralization, Human)
• JH Kim, SJ Jang, JW Yun, MH Jung, SH Woo Effects of pepsin and pepstatin on reflux tonsil hypertrophy in vitro PLoS ONE, 2018-11-08;13(11):e0207090. 2018-11-08 [PMID: 30408092] (Neutralization, Human)
• T Carmenate, Y Ortíz, M Enamorado, K García-Mar, J Avellanet, E Moreno, L Graça, K León Blocking IL-2 Signal In Vivo with an IL-2 Antagonist Reduces Tumor Growth through the Control of Regulatory T Cells J. Immunol., 2018-04-04;0(0):. 2018-04-04 [PMID: 29618524] (Bioassay, Human)
• S Revu, J Wu, M Henkel, N Rittenhous, A Menk, GM Delgoffe, AC Poholek, MJ McGeachy IL-23 and IL-1? Drive Human Th17 Cell Differentiation and Metabolic Reprogramming in Absence of CD28 Costimulation Cell Rep, 2018-03-06;22(10):2642-2653. 2018-03-06 [PMID: 29514093] (Neutralization, Human)
• Lauryn E. Klevorn, Melissa M. Berrien-Elliott, Jinyun Yuan, Lindsey M. Kuehm, Gregory D. Felock, Sean A. Crowe et al. Rescue of Tolerant CD8+ T Cells during Cancer Immunotherapy with IL2:Antibody Complexes Cancer Immunology Research 2016-12-01 [PMID: 27803062]
• Enhanced immune response of MAIT cells in tuberculous pleural effusions depends on cytokine signaling Sci Rep, 2016-09-02;6(0):32320. 2016-09-02 [PMID: 27586092] (Flow Cytometry, Neutralization, Human)
• Activin A programs the differentiation of human TFH cells Nat Immunol, 2016-07-04;17(8):976-84. 2016-07-04 [PMID: 27376469] (Bioassay, Human)
• Jürgen Knobloch, Sarah-Jane Chikosi, Sarah Yanik, Jan Rupp, David Jungck, Andrea Koch A systemic defect in Toll-like receptor 4 signaling increases lipopolysaccharide-induced suppression of IL-2-dependent T-cell proliferation in COPD American Journal of Physiology-Lung Cellular and Molecular Physiology 2016-01-01 [PMID: 26498252]
• Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. J Biol Chem, 2015-07-16;290(37):22338-51. 2015-07-16 [PMID: 26183780] (Neutralization, Human)
• Kim , Dae Won, Zloza , Andrew, Broucek , Joseph, Schenkel , Jason M, Ruby , Carl, Samaha , Georges, Kaufman , Howard L Interleukin-2 alters distribution of CD144 (VE-cadherin) in endothelial cells. J Transl Med, 2014-05-06;12(0):113. 2014-05-06 [PMID: 24885155] (Neutralization, Human)
• ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation. Blood, 2012-05-10;119(26):6268-77. 2012-05-10 [PMID: 22577174] (Neutralization, Human)
• Rathi L. Srinivas, Stephen C. Chapin, Patrick S. Doyle Aptamer-Functionalized Microgel Particles for Protein Detection Analytical Chemistry 2011-12-01 [PMID: 22017663]
• Hutnick NA, Carnathan D, Demers K, Makedonas G, Ertl HC, Betts MR Adenovirus-specific human T cells are pervasive, polyfunctional, and cross-reactive. Vaccine, 2010-02-23;28(8):1932-41. 2010-02-23 [PMID: 20188249] (Flow Cytometry, Human)
• Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue. Blood, 2007-02-13;109(11):4856-64. 2007-02-13 [PMID: 17299094] (Neutralization, Human)
• Karenko L, Hahtola S, Paivinen S, Karhu R, Syrja S, Kahkonen M, Nedoszytko B, Kytölä S, Zhou Y, Blazevic V, Pesonen M, Nevala H, Nupponen N, Sihto H, Krebs I, Poustka A, Roszkiewicz J, Saksela K, Peterson P, Visakorpi T, Ranki A Primary cutaneous T-cell lymphomas show a deletion or translocation affecting NAV3, the human UNC-53 homologue. Cancer Res., 2005-09-15;65(18):8101-10. 2005-09-15 [PMID: 16166283] (Flow Cytometry, ICC, Human)
• Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells. Immunology, 2004-06-01;112(2):274-89. 2004-06-01 [PMID: 15147571] (Flow Cytometry, Human)
• Herrero C, Hu X, Li WP, Samuels S, Sharif MN, Kotenko S, Ivashkiv LB Reprogramming of IL-10 activity and signaling by IFN-gamma. J. Immunol., 2003-11-15;171(10):5034-41. 2003-11-15 [PMID: 14607900] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • NP_000577()