IL-2 Antibody [Unconjugated]

IL-2 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

IL-2 was detected in immersion fixed bovine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Bovine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2465) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Bv
• Applications: WB, ELISA(Cap), ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant bovine IL‑2
  Ala21-Thr155
  Accession # P05016
• Specificity: Detects bovine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Bovine IL-2 Antibody (Catalog # AF2465)
  ELISA Detection:Bovine IL-2 Biotinylated Antibody (Catalog # BAF2465)
  Standard: Recombinant Bovine IL-2 (Catalog # 2465-BL)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 Antibody [Unconjugated]

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

Bovine Interleukin-2 (IL-2) is a 15 kDa, alpha -helical, single chain, potentially glycosylated polypeptide that has potent stimulatory activity for antigen-activated T cells (1‑5). The molecule is synthesized as a 155 amino acid (aa) precursor that contains a 20 aa signal peptide plus a 135 aa mature segment that is possibly O‑glycosylated (4, 5). The mature region has multiple alpha -helices and one intrachain disulfide bond. Mature bovine IL-2 is 64%, 60%, 49%, 50%, 72%, 63% and 67% to mature human, canine, mouse, rat, porcine, equine, and feline IL-2, respectively. Mammalian cells known to express IL-2 include CD4⁺ and CD8⁺ T cells, visceral smooth muscle cells, eosinophils, gamma δ T cells, B cells and dendritic cells. The receptor for IL-2 is complex and consists of three distinct subunits in varying combinations (6, 7). Two of these are ligand-binding and are termed IL-2 R alpha and IL-2 R beta . IL-2 R alpha is 55 kDa and binds IL-2 with low affinity. IL-2 R beta is 75 kDa and binds IL-2 with intermediate affinity. Signal transduction is performed by both IL-2 R beta and a 64 kDa common gamma chain ( gamma _c). This signal transducing common gamma chain does not bind IL-2, but does heterodimerize with IL-2 R beta to form a functional IL-2 receptor. The complex heterotrimeric alpha -beta -gamma c receptor may arise from IL-2 binding to preformed R alpha -R beta  complexes (8). Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells into active G1, inducing IL-2 and IL-2 R alpha synthesis and cell proliferation (7). It also promotes Fas-induced death of naïve CD4⁺ T cells, while having minimal effect on activated CD4⁺ memory lymphocytes. Finally, IL-2 seems to play a central role in the expansion and maintenance of CD4⁺ CD25⁺ regulatory T cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (9, 10).

1. Smith, K.A. (1992) Curr. Opin. Immunol. 4:271.
2. Smith, K.A. (1988) Science 240:1169.
3. Waldmann, T.A. et al. (2001) Immunity 14:105.
4. Cerretti, D.P. et al. (1986) Proc. Natl. Acad. Sci. USA 83:3223.
5. Reeves, R. et al. (1986) Proc. Natl. Acad. Sci. USA 83:3228.
6. Ellery, J.M. and P.J. Nicholls (2002) Cytokine Growth Factor Rev. 13:27.
7. Nelson, B.H. and D.M. Willerford (1998) Adv. Immunol. 70:1.
8. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
9. Jaleco, S. et al. (2003) J. Immunol. 171:61.
10. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.

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Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • P05016()