IL-2 R beta Antibody [Unconjugated]

Recombinant Human IL-2 (Catalog # 202-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-2 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-2 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-224-NA). The ND50 is typically 10‑30 µg/mL.

Simple Western lane view shows lysates of HDLM‑2 human Hodgkin’s lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IL‑2 R beta at approximately 98 kDa (as indicated) using 25 µg/mL of Goat Anti-Human IL‑2 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-224-NA). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-2 R beta Antibody [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
  Ala27-Asp239
  Accession # NP_000869
• Specificity: Detects human IL‑2 R beta in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with
  recombinant human (rh) IL-2 R alpha , recombinant mouse (rm) IL-2 R beta , rhIL-2 R gamma , and rhIL-15 R is observed. Is also able to block the cell surface of human IL-2 R beta mediated bioactivities induced by IL-2. For optimal neutralization of IL-2 biological activity on cells expressing the high affinity IL-2 receptors, the use of anti-IL-2 R alpha in conjunction with anti-IL-2 R beta antibodies is recommended.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL2RB
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 10-30 ug/mL
  • Simple Western 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-2 R beta Antibody [Unconjugated]

• CD122 antigen
• CD122
• high affinity IL-2 receptor beta subunit
• High affinity IL-2 receptor subunit beta
• IL-15 R beta
• IL-2 R beta
• IL-2 receptor subunit beta
• IL2R beta
• IL-2R subunit beta
• IL2RB
• IL-2Rb
• interleukin 15 receptor, beta
• interleukin 2 receptor, beta
• interleukin-2 receptor subunit beta
• P70-75
• p75

Background

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha , beta , and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling.

IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta ) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta . At present, the function of IL-2 sR beta is unclear. Recombinant human IL-2 sR beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, IL-2 sR beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-2 R beta Antibody (AF-224-NA)(2)

Publications using AF-224-NA

• Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients. J. Immunol., 2008-06-01;180(11):7221-9. 2008-06-01 [PMID: 18490721] (Western Blot, Human)
• Yamasaki S, Maeda M, Ohshima K, Kikuchi M, Otsuka T, Harada M Growth and apoptosis of human natural killer cell neoplasms: role of interleukin-2/15 signaling. Leuk. Res., 2004-10-01;28(10):1023-31. 2004-10-01 [PMID: 15289013] (Neutralization, Human)

Bioinformatics

• Gene Symbol: IL2RB
• Uniprot:
  • NP_000869()