Recombinant Mouse IL-2 Protein

SDS-Page: Mouse IL-2 Protein [NBP2-35111]

• Reactivity: Mu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Mouse IL-2 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 149 amino acids corresponding to IL-2 Source: E. coli

  Uniprot ID: P04351

  Amino Acid Sequence: APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ

• Details of Functionality: IL2 Protein is Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 x 10^6 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: IL2
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of IL-2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 17.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Mouse IL-2 Protein

• Aldesleukin
• IL2
• IL-2
• IL-2lymphokine
• interleukin 2
• interleukin-2
• involved in regulation of T-cell clonal expansion
• Proleukin
• T cell growth factor
• T-cell growth factor
• TCGF

Background

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, gammadelta T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for Tcell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 k Da IL-2Ralpha, 75 k Da IL-2Rbeta, 64 k Da common gamma chain gammac/IL2Rgamma) that are present on the cell surface in varying preformed complexes. Mature mouse IL-2 shares 56% and 73% amino acid sequence identity with human and rat IL-2 respectively. Mouse and human IL-2 exhibit cross-species activity. Mouse IL-2 has a specific N-terminal region that contains a poly glutamine (contain 12 glutamines) stretch.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for IL-2 Recombinant Protein (NBP2-35111)(1)

Publication using NBP2-35111

• Dou D, Liang J, Zhai X et al. Oxytocin signalling in dendritic cells regulates immune tolerance in the intestine and alleviates DSS-induced colitis Clinical science (London, England : 1979) 2021-02-26 [PMID: 33564880]

Bioinformatics

• Gene Symbol: IL2
• Uniprot:
  • P04351()
  • P04351()
  • P04351()