IL-23R Antibody (258010) [Unconjugated]

In the presence of Recombinant Mouse IL‑2 (10 ng/mL, Catalog # 402-ML), Recombinant Mouse IL‑23 (Catalog # 1887-ML) stimulates IL‑17 secretion in mouse splenocytes in a dose-dependent manner (orange line), as measured by the Mouse IL‑17 Quantikine ELISA Kit (Catalog # M1700). Under these conditions, IL‑17 secretion elicited by Recombinant Mouse IL‑23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑23 R Monoclonal Antibody (Catalog # MAB1686). The ND50 is typically 0.1-0.4 µg/mL.

• Reactivity: Mu
• Applications: WB, B/N
• Clone: 258010
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-23R Antibody (258010) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
  Gly24-Asp372
  Accession # Q8K4B4
• Specificity: Detects mouse IL-23 R in Western blots. In Western blots, this antibody does not cross-react with recombinant human IL-23 R.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: IL23R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.1-0.4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23R Antibody (258010) [Unconjugated]

• IBD17
• IL-23 R
• IL-23 receptor
• IL23R
• IL-23R
• interleukin 23 receptor
• interleukin-23 receptor

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4⁺ CD45RB^low memory T cells but at much lower levels by mouse CD4⁺ CD45RB^high cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5448.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23R Antibody (MAB1686)(12)

Publications using MAB1686

• Han, S;Kim, B;Hyeon, DY;Jeong, D;Ryu, J;Nam, JS;Choi, YH;Kim, BR;Park, SC;Chung, YW;Shin, SJ;Lee, JY;Kim, JK;Park, J;Lee, SW;Kim, TB;Cheon, JH;Cho, HJ;Kim, CH;Yoon, JH;Hwang, D;Ryu, JH; Distinctive CD39+CD9+ lung interstitial macrophages suppress IL-23/Th17-mediated neutrophilic asthma by inhibiting NETosis Nature communications 2024-10-04 [PMID: 39366998] (Bioassay, Mouse)
• Y Chen, C Shao, NW Fan, T Nakao, A Amouzegar, SK Chauhan, R Dana The functions of IL-23 and IL-2 on driving autoimmune effector T-helper 17 cells into the memory pool in dry eye disease Mucosal Immunol, 2020-04-23;0(0):. 2020-04-23 [PMID: 32327706] (in vivo blocking, Mouse)
• P Zarin, TSH In, ELY Chen, J Singh, GW Wong, M Mohtashami, DL Wiest, MK Anderson, JC Zúñiga-Pfl Integration of T Cell Receptor, Notch and Cytokine Signals Programs in Mouse ?? T Cell Effector Differentiation Immunol. Cell Biol., 2018-06-28;0(0):. 2018-06-28 [PMID: 29754419] (Neutralization, Mouse)
• TM Hummel, T Ackfeld, M Schönberg, G Ciupka, F Schulz, A Oberdoerst, J Grötzinger, J Scheller, DM Floss Synthetic deletion of the Interleukin 23 receptor (IL-23R) stalk region led to autonomous IL-23R homodimerization and activation Mol. Cell. Biol., 2017-08-11;0(0):. 8/11/2017 [PMID: 28630278]
• Tahseen H Nasti IL-23 Inhibits Melanoma Development by Augmenting DNA Repair and Modulating T Cell Subpopulations J. Immunol, 2016-12-21;0(0):. 2016-12-21 [PMID: 28003381] (Flow Cytometry, Mouse)
• Murphy A, O'Keeffe K, Lalor S, Maher B, Mills K, McLoughlin R Staphylococcus aureus infection of mice expands a population of memory gammadelta T cells that are protective against subsequent infection. J Immunol, 2014-03-12;192(8):3697-708. 2014-03-12 [PMID: 24623128] (Neutralization, Mouse)
• Jianqin He, Guanjing Lang, Shiping Ding, Lanjuan Li Pathological Role of Interleukin-17 in Poly I:C-Induced Hepatitis PLoS ONE 2013-09-19 [PMID: 24069246]
• Kumar P, Thakar M, Ouyang W, Malarkannan S IL-22 from conventional NK cells is epithelial regenerative and inflammation protective during influenza infection. Mucosal Immunol, 2012-06-27;6(1):69-82. 2012-06-27 [PMID: 22739232] (Flow Cytometry, Mouse)
• Ma Y, Aymeric L, Locher C, Mattarollo SR, Delahaye NF, Pereira P, Boucontet L, Apetoh L, Ghiringhelli F, Casares N, Lasarte JJ, Matsuzaki G, Ikuta K, Ryffel B, Benlagha K, Tesniere A, Ibrahim N, Dechanet-Merville J, Chaput N, Smyth MJ, Kroemer G, Zitvogel L Contribution of IL-17-producing gamma delta T cells to the efficacy of anticancer chemotherapy. J. Exp. Med., 2011-03-07;208(3):491-503. 2011-03-07 [PMID: 21383056] (Neutralization, Mouse)
• Wu S, Rhee KJ, Albesiano E, Rabizadeh S, Wu X, Yen HR, Huso DL, Brancati FL, Wick E, McAllister F, Housseau F, Pardoll DM, Sears CL A human colonic commensal promotes colon tumorigenesis via activation of T helper type 17 T cell responses. Nat. Med., 2009-08-23;15(9):1016-22. 2009-08-23 [PMID: 19701202] (Neutralization, Mouse)
• Harris TJ, Grosso JF, Yen HR, Xin H, Kortylewski M, Albesiano E, Hipkiss EL, Getnet D, Goldberg MV, Maris CH, Housseau F, Yu H, Pardoll DM, Drake CG Cutting edge: An in vivo requirement for STAT3 signaling in TH17 development and TH17-dependent autoimmunity. J. Immunol., 2007-10-01;179(7):4313-7. 2007-10-01 [PMID: 17878325] (Neutralization, Mouse)
• Shibata K, Yamada H, Hara H, Kishihara K, Yoshikai Y Resident Vdelta1+ gammadelta T cells control early infiltration of neutrophils after Escherichia coli infection via IL-17 production. J. Immunol., 2007-04-01;178(7):4466-72. 2007-04-01 [PMID: 17372004] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: IL23R
• Uniprot:
  • Q8K4B4()