IL-23R Antibody (753317) [Allophycocyanin]

Mouse splenocytes treated with 10 µg/mL Anti-CD3 (Catalog # MAB484), 5 µg/mL Anti-CD28 (Catalog # AF483), 10 ng/mL Recombinant Mouse TGF-beta 1 (Catalog # 100-B), 20 ng/mL Recombinant Mouse IL-23 (Catalog # 1887-ML), 40 ng/mL Recombinant Mouse IL-6 (Catalog # 406-ML), and 10 ng/mL Recombinant Mouse IL-1 beta (Catalog # 401-ML) for 5 days to induce T17 activation were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse IL-23 R APC-conjugated Monoclonal Antibody (Catalog # FAB16861A) or (B) Rat IgG1Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

HEK293 human embryonic kidney cell line transfected with (A) mouse IL-23 R or (B) irrelevant transfectants was stained with Rat Anti-Mouse IL-23 R APC-conjugated Monoclonal Antibody (Catalog # FAB16861A, filled histogram) or isotype control antibody (Catalog # IC005A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

IL-23R Antibody (753317) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse IL-23 R
  Gly24-Asp372
  Accession # Q8K4B4
• Specificity: Detects endogenous mouse IL-23 R by flow cytometry.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: IL23R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-23R Antibody (753317) [Allophycocyanin]

• IBD17
• IL-23 R
• IL-23 receptor
• IL23R
• IL-23R
• interleukin 23 receptor
• interleukin-23 receptor

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1‑5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Mouse IL-23 R cDNA encodes a 644 amino acid (aa) type I transmembrane protein with a 23 aa residue signal peptide, a 349 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 249 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Mouse IL-23 R is expressed in mouse Th1 and Th2 cells, bone marrow, dendritic cells and macrophages. It is also expressed by mouse CD4⁺ CD45RB^low memory T cells but at much lower levels by mouse CD4⁺ CD45RB^high cells. IL-23 initiates a signal transduction cascade similar to that of IL-12 and involves Jak2, Tyk2, STAT1, STAT3, STAT4, and STAT5. IL-23 has biological activities that are similar to, but distinct from, IL-12.

1. Oppmann, B. et al. (2000) Immunity 13:715.
2. Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
3. Parham, C. et al. (2002) J. Immunol. 168:5448.
4. Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
5. Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-23R Antibody (FAB16861A)(2)

Publications using FAB16861A

• N Millet, NV Solis, D Aguilar, MS Lionakis, RT Wheeler, N Jendzjowsk, M Swidergall IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis Nature Communications, 2022-09-22;13(1):5545. 2022-09-22 [PMID: 36138043] (Flow Cytometry, Mouse)
• Lee P, Yang Y, Racke M, Lovett-Racke A Analysis of TGF-beta1 and TGF-beta3 as regulators of encephalitogenic Th17 cells: Implications for multiple sclerosis. Brain Behav Immun, 2014-12-10;46(0):44-9. 2014-12-10 [PMID: 25499467] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: IL23R
• Uniprot:
  • Q8K4B4()