IL-6R alpha Antibody (17506) [Unconjugated]

Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

U937 human histiocytic lymphoma cell line was labeled with HumanIL-6R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgGF(ab')2 Secondary Antibody (Catalog # F0102B).

Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL‑6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL‑6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically0.04-0.2 µg/mL.

• Reactivity: Hu
• Applications: WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
• Clone: 17506
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

IL-6R alpha Antibody (17506) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
  Leu20-Asp339
  Accession # P08887
• Specificity: Detects human IL-6R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha , recombinant mouse (rm) IL-1 alpha , rhIL-1 beta , rmIL-1 beta , rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL6R
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Neutralization 0.04-0.2 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human IL-6 Ralpha Biotinylated Antibody (Catalog number BAF227)
  Standard: Recombinant Human IL-6 Ralpha (Catalog number 227-SR)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-6R alpha Antibody (17506) [Unconjugated]

• CD126
• gp80
• IL-6 R alpha
• IL6Q
• IL6R alpha
• IL-6R alpha
• IL6R
• IL-6R-1
• IL6RA
• IL-6Ra
• IL6RQ
• interleukin 6 receptor

Background

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

1. Yamasaki et al. (1988) Science 241:825.
2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
3. Hibi et al. (1990) Cell 63:1149.
4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
5. Novick et al. (1989) J. Exp. Med. 170:1409.
6. Yasukawa et al. (1990) J. Biochem. 108:673.
7. Saito et al. (1991) J. Immunology 147:168.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-6R alpha Antibody (MAB227)(32)

Publications using MAB227

• Kumar, B;Khatpe, AS;Guanglong, J;Batic, K;Bhat-Nakshatri, P;Granatir, MM;Addison, RJ;Szymanski, M;Baldridge, LA;Temm, CJ;Sandusky, G;Althouse, SK;Cote, ML;Miller, KD;Storniolo, AM;Nakshatri, H; Stromal heterogeneity may explain increased incidence of metaplastic breast cancer in women of African descent Nature communications 2023-09-14 [PMID: 37709737]
• S Shankar, J Stolp, SC Juvet, J Beckett, PS Macklin, F Issa, J Hester, KJ Wood Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 2022-06-03;13(1):3121. 2022-06-03 [PMID: 35660734] (Neutralization, Human)
• Karavyraki M, Porter RK. Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma Medical Oncology 2022-04-29 [PMID: 35484352]
• Kathrin Pollmann, Sarala Raj Murthi, Damir Kračun, Thomas Schwarzmayr, Andreas Petry, Julie Cleuziou et al. Molecular signaling pathways in right ventricular impairment of adult patients after tetralogy of Fallot repair Cardiovascular Diagnosis and Therapy 2021-12-01 [PMID: 35070799]
• S Sugahara, K Hanaoka, T Emori, N Takeshita, Y Fujii, M Nakano, T Suzuki, J Takahashi, Y Nakamura Peficitinib improves bone fragility by recovering bone turnover imbalance in arthritic mice Journal of pharmacological sciences, 2021-11-03;148(1):134-141. 2021-11-03 [PMID: 34924117] (Neutralization, Human)
• E Kim, H Um, J Park, JW Jung, JE Kim, H Lee, EA Shin, Y Pinanga, H Lee, SH Nam, JW Lee TM4SF5-dependent crosstalk between hepatocytes and macrophages to reprogram the inflammatory environment Cell Reports, 2021-11-16;37(7):110018. 2021-11-16 [PMID: 34788612] (Western Blot, Human)
• ML Kutys, WJ Polacheck, MK Welch, KA Gagnon, T Koorman, S Kim, L Li, AI McClatchey, CS Chen Uncovering mutation-specific morphogenic phenotypes and paracrine-mediated vessel dysfunction in a biomimetic vascularized mammary duct platform Nat Commun, 2020-07-06;11(1):3377. 7/6/2020 [PMID: 32632100]
• Barrow MA, Martin ME, Coffey A et al. A functional role for the cancer disparity-linked genes, CRY beta B2 and CRY beta B2P1, in the promotion of breast cancer Breast Cancer Res. 9/11/2019 [PMID: 31511085]
• Endre Kristóf, Ágnes Klusóczki, Roland Veress, Abhirup Shaw, Zsolt Sándor Combi, Klára Varga et al. Interleukin-6 released from differentiating human beige adipocytes improves browning Experimental Cell Research 2019-04-01 [PMID: 30794803] (Bioassay, Human)
• C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies J. Leukoc. Biol., 2018-07-24;0(0):. 2018-07-24 [PMID: 30040142] (Neutralization, Human)
• IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 2016-11-15;90(0):88-95. 2016-11-15 [PMID: 27863335] (Flow Cytometry, Human)
• Rusan Catar, Janusz Witowski, Nan Zhu, Christian Lücht, Alicia Derrac Soria, Javier Uceda Fernandez et al. IL-6 Trans–Signaling Links Inflammation with Angiogenesis in the Peritoneal Membrane Journal of the American Society of Nephrology 2017-04-01 [PMID: 27837150]
• Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium PLoS ONE, 2016-05-12;11(5):e0155161. 2016-05-12 [PMID: 27171357] (Neutralization, Human)
• Jokerst JV, Chen Z, Xu L et al. A Magnetic Bead-Based Sensor for the Quantification of Multiple Prostate Cancer Biomarkers. PLoS One 1/1/2015 [PMID: 26421725]
• Andrej Lissat, Mandy Joerschke, Dheeraj A. Shinde, Till Braunschweig, Angelina Meier, Anna Makowska et al. IL6 secreted by Ewing sarcoma tumor microenvironment confers anti-apoptotic and cell-disseminating paracrine responses in Ewing sarcoma cells BMC Cancer 2015-12-01 [PMID: 26215971]
• Sommer A, Fabri M Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. PLoS ONE, 2015-06-24;10(6):e0130395. 2015-06-24 [PMID: 26107738] (Neutralization, Human)
• Meher Majety, Leon P. Pradel, Manuela Gies, Carola H. Ries Fibroblasts Influence Survival and Therapeutic Response in a 3D Co-Culture Model PLOS ONE 2015-06-08 [PMID: 26053043]
• Komori M, Blake A, Greenwood M et al. Cerebrospinal fluid markers reveal intrathecal inflammation in progressive multiple sclerosis. Ann Neurol 7/1/2015 [PMID: 25808056]
• Resheq Y, Li K, Ward S, Wilhelm A, Garg A, Curbishley S, Blahova M, Zimmermann H, Jitschin R, Mougiakakos D, Mackensen A, Weston C, Adams D Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells. J Immunol, 2015-02-09;194(6):2578-86. 2015-02-09 [PMID: 25667417] (Neutralization, Human)
• Takeshi Nowatari, Soichiro Murata, Ken Nakayama, Naoki Sano, Takehito Maruyama, Reiji Nozaki et al. Sphingosine 1-phosphate has anti-apoptotic effect on liver sinusoidal endothelial cells and proliferative effect on hepatocytes in a paracrine manner in human Hepatology Research 2015-11-01 [PMID: 25371278]
• Becker , T M, Boyd , S C, Mijatov , B, Gowrishankar , K, Snoyman , S, Pupo , G M, Scolyer , R A, Mann , G J, Kefford , R F, Zhang , X D, Rizos , H Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor. Oncogene, 2013-03-04;33(9):1158-66. 2013-03-04 [PMID: 23455323] (Neutralization, Human)
• Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 2012-04-26;484(7395):514-8. 2012-04-26 [PMID: 22466287] (Neutralization, Human)
• Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Nat. Med., 2011-10-30;17(11):1498-503. 2011-10-30 [PMID: 22037646] (In Vivo, Human)
• Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Nat. Immunol., 2009-07-05;10(8):857-63. 2009-07-05 [PMID: 19578369] (Neutralization, Human)
• Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Cancer Res., 2009-01-01;69(1):329-37. 2009-01-01 [PMID: 19118018] (Flow Cytometry, Neutralization, Human)
• Kolliputi N, Waxman AB IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation. Am. J. Respir. Cell Mol. Biol., 2008-09-05;40(3):314-24. 2008-09-05 [PMID: 18776134] (Western Blot, Human)
• Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol., 2007-08-05;8(9):942-9. 2007-08-05 [PMID: 17676045] (Neutralization, Human)
• Ammit AJ, Moir LM, Oliver BG, Hughes JM, Alkhouri H, Ge Q, Burgess JK, Black JL, Roth M Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-08-25;292(1):L199-206. 2006-08-25 [PMID: 16936245] (Western Blot, Human)
• Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5. 2004-12-01 [PMID: 15585763] (ELISA Development, Human)
• Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J. Immunol., 2002-11-01;169(9):5244-51. 2002-11-01 [PMID: 12391243] (ELISA Development, Human)
• Erices A, Conget P, Rojas C, Minguell JJ Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Exp. Cell Res., 2002-10-15;280(1):24-32. 2002-10-15 [PMID: 12372336] (Flow Cytometry, Neutralization, Human)
• Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity, 2001-06-01;14(6):705-14. 2001-06-01 [PMID: 11420041] (ELISA Development, Flow Cytometry, Human)

Review for IL-6R alpha Antibody (MAB227) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 04/04/2020

Summary

• Application: Western Blot
• Sample Tested: Human liver cancer cell line (HuH7),MDA-MB-231
• Species: Human
• Lot: IQ0916021

Bioinformatics

• Gene Symbol: IL6R
• Uniprot:
  • P08887()