Recombinant Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007) His Protein

SDS-Page: Recombinant Influenza A Virus H5N1 Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007) His Protein [NBP1-99028] - 15% SDS-PAGE (3ug)

• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007) His Protein Summary

• Description: A recombinant protein with a C-Terminal His-tag and corresponding to the amino acids 17-338 of Influenza A (H5N1) Influenza A Hemagglutinin H5
  
  Source: Baculovirus Amino Acid Sequence: ADPMDQICIG YHANNSTEQV DTIMEKNVTV THAQDILEKT HNGKLCDLDG VKPLILRDCS VAGWLLGNPM CDEFINVPEW SYIVEKANPA NDLCYPGNFN DYEELKHLLS RINHFEKIQI IPKSSWSDHE ASSGVSSACP YQGVPSFFRN VVWLIKKNNT YPTIKRSYNN TNQEDLLILW GIHHSNDAAE QTKLYQNPTT YISVGTSTLN QRLVPKIATR SKVNGQSGRM DFFWTILKPN DAINFESNGN FIAPEYAYKI VKKGDSAIMK SEVEYGNCNT KCQTPIGAIN SSMPFHNIHP LTIGECPKYV KSNKLVLATG LRNSPLHHHH HH
• Specificity: Influenza A H5N1 Hemagglutinin (A/Vietnam/HN31242/2007)
• Source: Baculovirus
• Protein/Peptide Type: Recombinant Protein
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1ug of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 37.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Background

H5N1 is a subtype of the species Influenza A virus of the Influenzavirus A genus of the Orthomyxoviridae family. consists of single-stranded eight-segment negative-sense genomic RNAs, helical viral ribonucleoprotein (RNP) complexes (RNA segments NP, PB2, PB1 and PA), three viral envelope proteins (hemagglutinin [HA], neuraminidase [NA], and M2 ion channel), and a maxtir (M1) protein. Influenza A viruses are further classified into 16 HA (H1-H16) and 9 NA (N1-N9) serotypes based on the antigenic characteristics of HA and NA envelope glycoproteins. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

FAQs for Influenza A H5N1 Recombinant Protein (NBP1-99028). (Showing 1 - 1 of 1 FAQ).

1.  I would like to know this Hemaglutinin is produced in E coli. Is the protein without glicosilation . glicosilation is important immunogen, so what is the purpose to produce the protein without glicosilation ,Is it also usfull as antigen?
   • Yest, NBP1-99028 is a recombinant protein produced by E.coli. Therefore, we expect it to be without glycoslyation. Depending on the protein, glycosylation may be important. Some proteins do not require glycoslyation for it to be folded properly and/or be fully functional. E.coli production is more economical compared to using mammlian cell lines. In this case, NBP1-99028 is designed to be used for PAGE applications. As the protein is mainly used in a state that is denatured, it is not required to be structurally intact.